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Api g 1 ELISA 2.0 kit - Single plate (EPC-AG1-1)

  • Api g 1 ELISA 2.0 kit - Single plate (EPC-AG1-1)

ELISA 2.0 kit for measuring Apium gravolens allergen, Api g 1
Antibodies: 5D4/7C4
Standard Range: 200-0.39 ng/mL
Limit of Detection: 01.56 ng/mL
Background: OD<0.08 at 450nm
Coefficient of Determination: R-squared>0.98
Contents:

Plates: Pre-coated with anti-Api g 1 monoclonal antibody 5D4
Vial 1: (white top) Api g 1 allergen standard
Concentration: 2,000 ng/ml
Vial 2: (brown) anti-Api g 1 antibody 7C4
Vial 3: (blue top) Streptavidin-Peroxidase
Bottle 1: Wash buffer, (10x concentrate)
Bottle 2: Assay buffer, (10x concentrate)
Bottle 3: TMB developing substrate
Bottle 4: Stop solution (0.5N sulfuric acid)
Storage: The ELISA 2.0 kit should be stored at 2-8°C.
Expiry: See product insert.
Not Provided:

Type I ultrapure water or 18.2MΩ de-ionized water
Volumetric measuring equipment (e.g. serological pipette, graduated cylinder)
Clean containers for buffer and reagent preparation
Calibrated single and multi-channel micropipettes and tips
Vortex mixer
Plate reader capable of reading absorbance at 450nm
Analysis software (recommended, but not required)
Notes: The allergen standard is recommended for immunoassay calibration
purposes only.
A list of frequently asked questions and troubleshooting guide can be found under the ‘Support’ tab on our website.
Product Resources:

EPC-AG1-1 Certificate of Analysis

Validation Performance Data

For research and commercial use in vitro: not for human in vivo or therapeutic use.
  Certificate of Analysis
Pre-coated Plate: 96-well polystyrene microtiter plate coated with
monoclonal antibody 5D4 and treated with stabilizing
agent. Sealed in foil pouch with desiccant.
Monoclonal Antibody: 5D4
Immunogen: Api g 1
Isotype: Mouse IgG1/kappa
Specificity: Binds to species specific epitope present on Apium gravolens allergen, Api g 1.
Purification: Produced in vitro cell culture and purified by affinity chromatography using Protein A. Single heavy and light chain bands on SDS-PAGE.
  Certificate of Analysis
Detection Antibody: 7C4
Immunogen: Api g 1
Isotype Mouse IgG1/kappa
Specificity: Binds to species specific epitope present on Apium gravolens allergen, Api g 1.
Purification: Produced in vitro cell culture and purified by affinity chromatography using Protein G. Single heavy and light chain bands on SDS-PAGE.
Activity: Titrated for use in ELISA at 1/1000 dilution. Prepared in 1% BSA/50% glycerol/PBS, pH 7.4, 0.22 μm filtered, preservative free.
  Certificate of Analysis
Allergen Standard: Purified recombinant Api g 1 allergen 1.0101 prepared in 1% BSA/50% glycerol/PBS, pH 7.4.
Concentration/Calibration: 2,000 ng/mL (based on amino acid analysis)
References:  
 
 
 

 

 
File Name Size Added Link
EPC-AG1 Api g 1 COA 1.17MB 22/06/2023 Download
EPC-AG1 Api g 1 COA
  • Product Code: EPC-AG1-1
  • Availability: In Stock

  • $595.00

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Tags: Api g 1, ELISA 2.0, pre-coated plate,