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Ara h 3 ELISA 2.0 kit - Single plate (EPC-AH3-1)

  • Ara h 3 ELISA 2.0 kit - Single plate (EPC-AH3-1)

ELISA 2.0 kit for measuring Arachis hypogaea allergen, Ara h 3.
Antibodies: 1E8/4G9
Standard Range: 125-0.49 ng/mL
Limit of Detection: 1.9ng/mL
Background: OD<0.08 at 450nm
Coefficient of Determination: R-squared>0.98
Contents:

Plate: Pre-coated with anti-Ara h 3 monoclonal antibody 1E8
Vial 1: (white top) Ara h 3 allergen standard
Concentration: 1250 ng/ml
Vial 2: (brown) anti-Ara h 3 antibody 4G9
Vial 3: (blue top) Streptavidin-Peroxidase
Bottle 1: Wash buffer, (10x concentrate)
Bottle 2: Assay buffer, (10x concentrate)
Bottle 3: TMB developing substrate
Bottle 4: Stop solution (0.5N sulfuric acid)
Storage: The ELISA 2.0 kit should be stored at 2-8°C.
Expiry: See product insert.
Not Provided:

Type I ultrapure water or 18.2MΩ de-ionized water
Volumetric measuring equipment (e.g. serological pipette, graduated cylinder)
Clean containers for buffer and reagent preparation
Calibrated single and multi-channel micropipettes and tips
Vortex mixer
Plate reader capable of reading absorbance at 450nm
Analysis software (recommended, but not required)
Notes: The allergen standard is recommended for immunoassay calibration
purposes only.
A list of frequently asked questions and troubleshooting guide can be found under the ‘Support’ tab on our website.
Product Resources:

EPC-AH3-1 Certificate of Analysis

Validation Performance Data

For research and commercial use in vitro: not for human in vivo or therapeutic use.
  Certificate of Analysis
Pre-coated Plate: 96-well polystyrene microtiter plate coated with
monoclonal antibody 1E8 and treated with stabilizing
agent. Sealed in foil pouch with desiccant.
Monoclonal Antibody: 1E8
Immunogen: Ara h 3
Isotype: Mouse IgG1
Specificity: Binds to an epitope on peanut allergen, Arachis hypogaea, Ara h 3.
Purification: Produced in tissue culture and purified by affinity chromatography using Protein A. Single heavy and light chain bands on SDS-PAGE.
  Certificate of Analysis
Detection Antibody: 4G9
Immunogen: Ara h 3
Isotype Mouse IgG1
Specificity: Binds to an epitope on peanut allergen, Arachis hypogaea, Ara h 3.
Activity: Biotinylated and titrated for use in ELISA at 1/1000 dilution. Prepared in 1% BSA/50% glycerol/PBS, pH 7.4, 0.22 μm filtered, preservative free.
  Certificate of Analysis
Allergen Standard: Purified natural Ara h 3 prepared in 1% BSA/50%
glycerol/PBS, pH 7.4.
Concentration/Calibration: 1,250 ng/mL (based on amino acid analysis)
References:  
1.  Perry TT, Conover-Walker MK, Pomés A, Chapman MD, Wood RA. Distribution of peanut allergen in the environment. J Allergy Clin Immunol. 2004 May;113(5):973-6.
2.  Maloney JM, Chapman MD, Sicherer SH. Peanut allergen exposure through saliva: assessment and interventions to reduce exposure. J Allergy Clin Immunol. 2006 Sep;118(3):719-24.
 

 

 
File Name Size Added Link
EPC-AH3-X_CoA 268.91KB 28/04/2020 Download
Certificate of Analysis for Ara h 3 ELISA 2.0 kit
  • Product Code: EPC-AH3-1
  • Availability: In Stock

  • $545.00

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Tags: Ara h 3, ELISA 2.0, pre-coated plate,