Ara h 6 ELISA 2.0 kit - Single plate (EPC-AH6-1)

Description
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ELISA 2.0 kit for measuring Arachis hypogaea allergen, Ara h 6.
Antibodies:		3B8/3E12
Standard Range:		25-0.05ng/mL
Limit of Detection:		0.2ng/mL
Background:		OD<0.08ng/ml at 450nm
Coefficient of Determination:		R-squared>0.98
Contents:		Plate: Pre-coated with anti-Ara h 6 monoclonal antibody 3B8 Vial 1: (white top) Ara h 6 allergen standard Concentration: 250 ng/ml Vial 2: (brown) anti-Ara h 6 antibody 3E12 Vial 3: (blue top) Streptavidin-Peroxidase Bottle 1: Wash buffer, (10x concentrate) Bottle 2: Assay buffer, (10x concentrate) Bottle 3: TMB developing substrate Bottle 4: Stop solution (0.5N sulfuric acid)
Storage:		The ELISA 2.0 kit should be stored at 2-8°C.
Expiry:		See product insert.
Not Provided:		Type I ultrapure water or 18.2MΩ de-ionized water Volumetric measuring equipment (e.g. serological pipette, graduated cylinder) Clean containers for buffer and reagent preparation Calibrated single and multi-channel micropipettes and tips Vortex mixer Plate reader capable of reading absorbance at 450nm Analysis software (recommended, but not required)
Notes:		The allergen standard is recommended for immunoassay calibration purposes only. A list of frequently asked questions and troubleshooting guide can be found under the ‘Support’ tab on our website.
Product Resources:		EPC-AH6-1 Certificate of Analysis Validation Performance Data
For research and commercial use in vitro: not for human in vivo or therapeutic use.
		Certificate of Analysis
Pre-coated Plate:		96-well polystyrene microtiter plate coated with monoclonal antibody 3B8 and treated with stabilizing agent. Sealed in foil pouch with desiccant.
Monoclonal Antibody:		3B8
Immunogen:		Ara h 6
Isotype:		Mouse IgG1
Specificity:		Binds to an epitope on peanut allergen, Arachis hypogaea, Ara h 6.
Purification:		Produced in ascites and purified by chromatography using Protein G. Single heavy and light chain bands on SDS-PAGE.

		Certificate of Analysis
Detection Antibody:		3E12
Immunogen:		Ara h 6
Isotype		Mouse IgG1
Specificity:		Binds to an epitope on peanut allergen, Arachis hypogaea, Ara h 6.
Activity:		Biotinylated and titrated for use in ELISA at 1/1000 dilution. Prepared in 1% BSA/50% glycerol/PBS, pH 7.4, 0.22 μm filtered, preservative free.

		Certificate of Analysis
Allergen Standard:		Purified natural Ara h 6 prepared in 1% BSA/50% glycerol/PBS, pH 7.4.
Concentration/Calibration:		250ng/mL (based on amino acid analysis)
References:
1. Perry TT, Conover-Walker MK, Pomés A, Chapman MD, Wood RA. Distribution of peanut allergen in the environment. J Allergy Clin Immunol. 2004 May;113(5):973-6.
2. Maloney JM, Chapman MD, Sicherer SH. Peanut allergen exposure through saliva: assessment and interventions to reduce exposure. J Allergy Clin Immunol. 2006 Sep;118(3):719-24.