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Can s 5 ELISA 2.0 kit - Five plate (EPC-CS5-5)

  • Can s 5 ELISA 2.0 kit - Five plate (EPC-CS5-5)

ELISA 2.0 kit for measuring Cannabis sativa allergen, Can s 5.

Antibodies: pAb/pAb
Standard Range: 50-0.10ng/mL
Limit of Detection: 0.39ng/mL
Background: OD<0.08 at 450nm
Coefficient of Determination: R-squared>0.98
Contents:

Plate: Pre-coated with anti-Can s 5 purified polyclonal antiserum
Vial 1: (white top) Can s 5 allergen standard
Concentration: 500ng/ml
Vial 2: (brown) Biotinylated purified polyclonal antiserum
Vial 3: (blue top) Streptavidin-peroxidase
Bottle 1: Wash buffer, (10x concentrate)
Bottle 2: Assay buffer, (10x concentrate)
Bottle 3: TMB developing substrate
Bottle 4: Stop solution (0.5N sulfuric acid)

Storage: Store at 2-8°C.
Expiry: See product insert.
Not Provided:

Type I ultrapure water or 18.2MΩ de-ionized water
Volumetric measuring equipment (e.g. serological pipette, graduated cylinder)
Clean containers for buffer and reagent preparation
Calibrated single and multi-channel micropipettes and tips
Vortex mixer
Plate reader capable of reading absorbance at 450nm
Analysis software (recommended, but not required)

Notes: The allergen standard is recommended for immunoassay calibration purposes only.
A list of frequently asked questions and troubleshooting guide can be found under the ‘Support’ tab on our website.
Product Resources:

EPC-CS5 Certificate of Analysis

Validation Performance Data

For research and commercial use in vitro: not for human in vivo or therapeutic use.

  Certificate of Analysis
Pre-coated Plate: 96-well polystyrene microtiter plate coated with
purified polyclonal antibody and treated with stabilizing
agent. Sealed in foil pouch with desiccant.
Polyclonal Antibody: Purified rabbit polyclonal IgG
Immunogen: Can s 5
Isotype: Multiple
Specificity: Binds to an epitope present on Cannabis sativa (hemp) allergen, Can s 5.
Purification: Antiserum purified by affinity chromatography. Single heavy and light chain bands on SDS-PAGE.

Detection Antibody: Purified rabbit polyclonal IgG
Immunogen: Can s 5
Isotype: Multiple
Specificity: Binds to an epitope present on Cannabis sativa (hemp) allergen, Can s 5.
Purification: Antiserum purified by affinity chromatography. Single heavy and light chain bands on SDS-PAGE.
Biotinylation: Biotinylated and titrated for use in ELISA at 1/1000 dilution. Prepared in 1% BSA/50% glycerol/PBS, pH 7.4, 0.22µm filtered, preservative free.

Allergen Standard: Recombinant Can s 5 prepared in 1% BSA/50%
glycerol/PBS, pH 7.4.
Concentration: 500ng/mL (based on amino acid analysis)
References:    
 
 

 

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  • Product Code: EPC-CS5-5
  • Availability: In Stock
  • $1,985.00

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