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Der f 2 ELISA 2.0 kit - Twenty-five plate (EPC-DF2-25)
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ELISA 2.0 kit for measuring Dermatophagoides spp. Group 2 allergen, Der f 2 *Not recommended for allergen quantification in samples that contain multiple house dust mite species |
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| Antibodies: | 1D8/7A1 | |||||
| Standard Range: | 250-0.49ng/mL | |||||
| Limit of Detection: | 0.98ng/mL | |||||
| Background: | OD<0.08 at 450nm | |||||
| Coefficient of Determination: | R-squared>0.98 | |||||
| Contents: | Plate: Pre-coated with anti-Der p 2 monoclonal antibody 1D8 Vial 1: (white top) Der f 2 allergen standard Concentration: 2,500ng/ml Vial 2: (brown) Biotinylated monoclonal antibody 7A1 Vial 3: (blue top) Streptavidin-peroxidase Bottle 1: Wash buffer, (10x concentrate) Bottle 2: Assay buffer, (10x concentrate) Bottle 3: TMB developing substrate Bottle 4: Stop solution (0.5N sulfuric acid) |
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| Storage: | Store at 2-8°C. | |||||
| Expiry: | See product insert. | |||||
| Not Provided: | Type I ultrapure water or 18.2MΩ de-ionized water Volumetric measuring equipment (e.g. serological pipette, graduated cylinder) Clean containers for buffer and reagent preparation Calibrated single and multi-channel micropipettes and tips Vortex mixer Plate reader capable of reading absorbance at 450nm Analysis software (recommended, but not required) |
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| Notes: | The allergen standard is recommended for immunoassay calibration purposes only. A list of frequently asked questions and troubleshooting guide can be found under the ‘Support’ tab on our website. |
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| Product Resources: | ||||||
For research and commercial use in vitro: not for human in vivo or therapeutic use. |
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| Certificate of Analysis | ||||||
| Pre-coated Plate: | 96-well polystyrene microtiter plate coated with monoclonal antibody 1D8 and treated with stabilizing agent. Sealed in foil pouch with desiccant. |
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| Monoclonal Antibody: | 1D8 (clone 1D8 C4 D8) | |||||
| Immunogen: | Der p 2 | |||||
| Isotype: | Mouse IgG2A | |||||
| Specificity: | Binds to a common epitope on mite Dermatophagoides spp. Group 2 allergens, Der p 2 and Der f 2. | |||||
| Purification: | From ascites by ammonium sulphate precipitation and purified by affinity chromatography using Protein A. Single heavy and light chain bands on SDS-PAGE. | |||||
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| Detection Antibody: | 7A1 (clone 7A1 H1 G3) | |||||
| Vial: | Brown Cap; 100µl | |||||
| Immunogen: | Der f 2 | |||||
| Isotype: | Mouse IgG1 | |||||
| Specificity: | Binds to a common epitope on mite Dermatophagoides spp Group 2 allergens (Der f 2 and Der p 2). | |||||
| Purification: | Produced in ascites and purified by affinity chromatography using Protein A. Single heavy and light chain bands on SDS-PAGE. | |||||
| Biotinylation: | Biotinylated and titrated for use in ELISA at 1/1000 dilution. Prepared in 1% BSA/50% glycerol/PBS, pH 7.4, 0.22µm filtered, preservative free. | |||||
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| Allergen Standard: | Purified natural Der f 2 prepared in 1% BSA/50% glycerol/PBS, pH 7.4. |
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| Concentration: | 2,500ng/mL (based on amino acid analysis) | |||||
| References: | ||||||
| 1. Luczynska CM, Arruda LK, Platts-Mills TA, Miller JD, Lopez M, Chapman MD. A two-site monoclonal antibody ELISA for the quantification of the major Dermatophagoides spp. allergens, Der p I and Der f I. J Immunol Methods 1989; 118(2):227-235. | ||||||
| 2. Custovic A, Taggart SC, Francis HC, Chapman MD, Woodcock A. Exposure to house dust mite allergens and the clinical activity of asthma. J Allergy Clin Immunol 1996; 98(1):64-72. | ||||||
| 3. Platts-Mills TA, Vervloet D, Thomas WR, Aalberse RC, Chapman MD. Indoor allergens and asthma: report of the Third International Workshop. J Allergy Clin Immunol 1997; 100(6 Pt 1):S2-24. | ||||||
| 4. Sawyer G, Kemp T, Shaw R et al. Biologic pollution in infant bedding in New Zealand: high allergen exposure during a vulnerable period. J Allergy Clin Immunol 1998; 102(5):765-770. | ||||||
| 5. Arbes SJ, Jr., Cohn RD, Yin M et al. House dust mite allergen in US beds: results from the First National Survey of Lead and Allergens in Housing. J Allergy Clin Immunol 2003; 111(2):408-414. | ||||||
| 6. Gruchalla RS, Pongracic J, Plaut M et al. Inner City Asthma Study: relationships among sensitivity, allergen exposure, and asthma morbidity. J Allergy Clin Immunol 2005; 115(3):478-485. | ||||||
| 7. Zock JP, Heinrich J, Jarvis D et al. Distribution and determinants of house dust mite allergens in Europe: the European Community Respiratory Health Survey II. J Allergy Clin Immunol 2006; 118(3):682-690. | ||||||
| 8. Jarvis D, Zock JP, Heinrich J et al. Cat and dust mite allergen levels, specific IgG and IgG(4), and respiratory symptoms in adults. J Allergy Clin Immunol 2007; 119:697-704. | ||||||
| 9. Earle CD, King EM, Tsay A et al. High-throughput fluorescent multiplex array for indoor allergen exposure assessment. J Allergy Clin Immunol 2007; 119(2):428-433. | ||||||
| File Name | Size | Added | Link |
| EPC-DF2-X COA | 1.15MB | 22/06/2023 | Download |
| EPC-DF2-X COA | |||
- Product Code: EPC-DF2-25
- Availability: In Stock
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$9,095.00
Tags: der f 2, der f 2, df2, ELISA 2.0, dust mite, pre-coated plate, group 2
