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Ory c 3 ELISA 2.0 kit - Single plate (EPC-OC3-1)

  • Ory c 3 ELISA 2.0 kit - Single plate (EPC-OC3-1)

ELISA 2.0 kit for measuring Oryctolagus cuniculus allergen, Ory c 3.
Antibodies: pAb/pAb
Standard Range: 20-0.04 ng/mL
Limit of Detection: 0.08ng/mL
Background: OD<0.08 at 450nm
Coefficient of Determination: R-squared>0.98
Contents:

Plate: Pre-coated with anti-Ory c 3 purified polyclonal goat antiserum.
Vial 1: (white top) Ory c 3 allergen standard
Concentration: 200ng/ml
Vial 2: (brown) Biotinylated purified polyclonal goat anti-Ory c 3 antiserum.
Vial 3: (blue top) Streptavidin-peroxidase
Bottle 1: Wash buffer, (10x concentrate)
Bottle 2: Assay buffer, (10x concentrate)
Bottle 3: TMB developing substrate
Bottle 4: Stop solution (0.5N sulfuric acid)
Storage: Store at 2-8°C
Expiry: See product insert.
Not Provided:

Type I ultrapure water or 18.2MΩ de-ionized water
Volumetric measuring equipment (e.g. serological pipette, graduated cylinder)
Clean containers for buffer and reagent preparation
Calibrated single and multi-channel micropipettes and tips
Vortex mixer
Plate reader capable of reading absorbance at 450nm
Analysis software (recommended, but not required)
Notes: The allergen standard is recommended for immunoassay calibration purposes only.
A list of frequently asked questions and troubleshooting guide can be found under the ‘Support’ tab on our website.
Product Resources:

EPC-OC3-1 Certificate of Analysis

Validation Performance Data

For research and commercial use in vitro: not for human in vivo or therapeutic use.
   
  Certificate of Analysis
Pre-coated Plate: 96-well polystyrene microtiter plate coated with
purified rabbit polyclonal antiserum and treated with stabilizing
agent. Sealed in foil pouch with desiccant.
Coating Antibody: Purified rabbit polyclonal antiserum
Immunogen: Ory c 3
Isotype: Multiple
Specificity: Binds to an epitope on rabbit Oryctolagus cuniculus allergen, Ory c 3.
Purification: Produced in vivo and purified by affinity chromatography. Single heavy and light chain bands on SDS-PAGE.
  Certificate of Analysis
Detection Antibody: Rabbit polyclonal antiserum
Immunogen: Ory c 3
Isotype: Multiple
Specificity: Binds to an epitope on rabbit Oryctolagus cuniculus allergen, Ory c 3.
Purification: Produced in vivo and purified by Affinity chromatography. Single heavy and light chain bands on SDS-PAGE.
Biotinylation: Biotinylated and titrated for use in ELISA at 1/1000 dilution. Prepared in 1% BSA/50% glycerol/PBS, pH 7.4, 0.22μm filtered, preservative free.
  Certificate of Analysis
Allergen Standard: Recombinant Ory c 3 prepared in 1% BSA/50% glycerol/PBS, pH 7.4.
Concentration: 200ng/mL (based on amino acid analysis)

References: 
 

 

File Name Size Added Link
EPC-OC3-X Ory c 3 COA 1.01MB 22/06/2023 Download
EPC-OC3-X Ory c 3 COA
  • Product Code: EPC-OC3-1
  • Availability: In Stock

  • $545.00

Tags: Ory c 3, ELISA 2.0, pre-coated plate, rabbit allergen,