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Pru du 6 (Almond) ELISA 2.0 kit - Single plate (EPC-PD6-1)

  • Pru du 6 (Almond) ELISA 2.0 kit - Single plate (EPC-PD6-1)

ELISA 2.0 kit for measuring Prunus dulcis (almond) allergen, Pru du 6
Antibodies: 6C10/1D3
Standard Range: 125-0.24 ng/mL
Limit of Detection: 0.49 ng/mL
Background: OD<0.08 at 450nm
Coefficient of Determination: R-squared>0.98
Contents:

Plates: Pre-coated with anti-Pru du 6 monoclonal antibody 6C10
Vial 1: (white top) Pru du 6 allergen standard
Concentration: 1,250 ng/mL
Vial 2: (brown) anti-Pru du 6 antibody 1D3
Vial 3: (blue top) Streptavidin-Peroxidase
Bottle 1: Wash buffer, (10x concentrate)
Bottle 2: Assay buffer, (10x concentrate)
Bottle 3: TMB developing substrate
Bottle 4: Stop solution (0.5N sulfuric acid)
Storage: The ELISA 2.0 kit should be stored at 2-8°C.
Expiry: See product insert.
Not Provided:

Type I ultrapure water or 18.2MΩ de-ionized water
Volumetric measuring equipment (e.g. serological pipette, graduated cylinder)
Clean containers for buffer and reagent preparation
Calibrated single and multi-channel micropipettes and tips
Vortex mixer
Plate reader capable of reading absorbance at 450nm
Analysis software (recommended, but not required)
Notes: The allergen standard is recommended for immunoassay calibration
purposes only.
A list of frequently asked questions and troubleshooting guide can be found under the ‘Support’ tab on our website.
Product Resources:

EPC-PD6-1 Certificate of Analysis

Validation Performance Data

For research and commercial use in vitro: not for human in vivo or therapeutic use.
  Certificate of Analysis
Pre-coated Plate: 96-well polystyrene microtiter plate coated with
monoclonal antibody 6C10 and treated with stabilizing
agent. Sealed in foil pouch with desiccant.
Monoclonal Antibody: 6C10
Immunogen: Pru du 6
Isotype: Mouse IgG1/kappa
Specificity: Binds to species specific epitope present on Prunus dulcis allergen, Pru du 6.
Purification: Produced in vitro cell culture and purified by affinity chromatography using Protein A. Single heavy and light chain bands on SDS-PAGE.
  Certificate of Analysis
Detection Antibody: 1D3
Immunogen: Pru du 6
Isotype Mouse IgG1/kappa
Specificity: Binds to species specific epitope present on Prunus dulcis allergen, Pru du 6.
Purification: Produced in vitro cell culture and purified by affinity chromatography using Protein A. Single heavy and light chain bands on SDS-PAGE.
Activity: Titrated for use in ELISA at 1/1000 dilution. Prepared in 1% BSA/50% glycerol/PBS, pH 7.4, 0.22 μm filtered, preservative free.
  Certificate of Analysis
Allergen Standard: Purified natural Pru du 6 allergen prepared in 1% BSA/50% glycerol/PBS, pH 7.4.
Concentration/Calibration: 1,250 ng/mL (based on amino acid analysis)
References:  
 
 
 

 

 
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  • Product Code: EPC-PD6-1
  • Availability: In Stock

  • $545.00

Tags: Pru du 6, ELISA 2.0, pre-coated plate, EPC-PD6, almond