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Ses i 1 ELISA 2.0 kit - Single plate (EPC-SI1-1)

  • Ses i 1 ELISA 2.0 kit - Single plate (EPC-SI1-1)

ELISA 2.0 kit for measuring Sesamum indicum allergen, Ses i 1
Antibodies: pAb/pAb
Standard Range: 25-0.05 ng/mL
Limit of Detection: 0.39 ng/mL
Background: OD<0.08 at 450nm
Coefficient of Determination: R-squared>0.98
Contents:

Plates: Pre-coated with polyclonal rabbit anti-Ses i 1 antibody
Vial 1: (white top) Ses i 1 allergen standard
Concentration: 250 ng/mL
Vial 2: (brown) biotinylated polyclonal rabbit anti-Ses i 1 antibody
Vial 3: (blue top) Streptavidin-Peroxidase
Bottle 1: Wash buffer, (10x concentrate)
Bottle 2: Assay buffer, (10x concentrate)
Bottle 3: TMB developing substrate
Bottle 4: Stop solution (0.5N sulfuric acid)
Storage: The ELISA 2.0 kit should be stored at 2-8°C.
Expiry: See product insert.
Not Provided:

Type I ultrapure water or 18.2MΩ de-ionized water
Volumetric measuring equipment (e.g. serological pipette, graduated cylinder)
Clean containers for buffer and reagent preparation
Calibrated single and multi-channel micropipettes and tips
Vortex mixer
Plate reader capable of reading absorbance at 450nm
Analysis software (recommended, but not required)
Notes: The allergen standard is recommended for immunoassay calibration
purposes only.
A list of frequently asked questions and troubleshooting guide can be found under the ‘Support’ tab on our website.
Product Resources:

EPC-SI1-1 Certificate of Analysis

Validation Performance Data

For research and commercial use in vitro: not for human in vivo or therapeutic use.
  Certificate of Analysis
Pre-coated Plate: 96-well polystyrene microtiter plate coated with
polyclonal antibody and treated with stabilizing
agent. Sealed in foil pouch with desiccant.
Polyclonal Antibody: Purified rabbit polyclonal IgG
Immunogen: Ses i 1
Isotype: Multiple
Specificity: Binds to an epitope on sesame allergen, Sesamum indicum, Ses i 1.
Purification: Produced in vivo and purified by affinity chromatography. Single heavy and light chain bands on SDS-PAGE.
  Certificate of Analysis
Detection Antibody: Biotinylated purified rabbit polyclonal IgG
Immunogen: Ses i 1
Isotype Multiple
Specificity: Binds to an epitope on sesame allergen, Sesamum indicum, Ses i 1.
Purification: Produced in vivo and purified by affinity chromatography. Single heavy and light chain bands on SDS-PAGE.
Activity: Titrated for use in ELISA at 1/1000 dilution. Prepared in 1% BSA/50% glycerol/PBS, pH 7.4, 0.22 μm filtered, preservative free.
  Certificate of Analysis
Allergen Standard: Purified recombinant Ses i 1 allergen 1.0101 prepared in 1% BSA/50% glycerol/PBS, pH 7.4.
Concentration/Calibration: 250 ng/mL (based on amino acid analysis)
References:  
 
 
 

 

 
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  • Product Code: EPC-SI1-1
  • Availability: In Stock

  • $595.00

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Tags: Ses i 1, ELISA 2.0, pre-coated plate,