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Tyr p 2 ELISA 2.0 kit - Single plate (EPC-TP2-1)

  • Tyr p 2 ELISA 2.0 kit - Single plate (EPC-TP2-1)

ELISA 2.0 kit for measuring Tyrophagus putrescentiae allergen, Tyr p 2.
Antibodies: pAb/pAb
Standard Range: 500-0.98 ng/mL
Limit of Detection: 7.8ng/mL
Background: OD<2.0 at 450nm
Coefficient of Determination: R-squared>0.98
Contents:

Plate: Pre-coated with purified anti-Tyr p 2 polyclonal rabbit capture
antibody.
Vial 1: (white top) Tyr p 2 allergen standard
Concentration: 5,000 ng/ml
Vial 2: (brown) Biotinylated purified polyclonal rabbit anti-Tyr p 2 antibody.
Vial 3: (blue top) Streptavidin-peroxidase
Bottle 1: Wash buffer, (10x concentrate)
Bottle 2: Assay buffer, (10x concentrate)
Bottle 3: TMB developing substrate
Bottle 4: Stop solution (0.5N sulfuric acid)
Storage: Store at 2-8°C
Expiry: See product insert.
Not Provided:

Type I ultrapure water or 18.2MΩ de-ionized water
Volumetric measuring equipment (e.g. serological pipette, graduated cylinder)
Clean containers for buffer and reagent preparation
Calibrated single and multi-channel micropipettes and tips
Vortex mixer
Plate reader capable of reading absorbance at 450nm
Analysis software (recommended, but not required)
Notes: The allergen standard is recommended for immunoassay calibration
purposes only.
A list of frequently asked questions and troubleshooting guide can be found under the ‘Support’ tab on our website.
Product Resources:

EPC-TP2-1 Certificate of Analysis

Validation Performance Data

For research and commercial use in vitro: not for human in vivo or therapeutic use.
   
  Certificate of Analysis
Pre-coated Plate: 96-well polystyrene microtiter plate coated with purified rabbit polyclonal antiserum and treated with stabilizing
agent. Sealed in foil pouch with desiccant.
Coating Antibody: Purified rabbit polyclonal antiserum
Immunogen: Tyr p 2
Isotype: Multiple
Specificity: Binds to an epitope on mold storage mite Tyrophagus putrescentiae protein allergen, Tyr p 2.
Purification: Affinity chromatography using recombinant Protein G. Single heavy and light chain bands on SDS-PAGE.
  Certificate of Analysis
Detection Antibody: Purified rabbit polyclonal antiserum
Immunogen: Tyr p 2
Isotype: Multiple
Specificity: Binds to an epitope on mold storage mite Tyrophagus putrescentiae protein allergen.
Purification: Affinity chromatography using recombinant Protein G. Single heavy and light chain bands on SDS-PAGE.
Biotinylation: Biotinylated and titrated for use in ELISA at 1/1000 dilution. Prepared in 1% BSA/50% glycerol/PBS, pH 7.4, 0.22μm filtered, preservative free.
  Certificate of Analysis
Allergen Standard: Recombinant Tyr p 2 prepared in 1% BSA/30% glycerol/PBS, pH 7.4.
Concentration: 5,000 ng/mL (based on advanced protein analysis)

References: 
 

 

File Name Size Added Link
EPC-TP2-X_CoA 203.8KB 28/04/2020 Download
Certificate of Analysis for Tyr p 2 ELISA 2.0 kit
  • Product Code: EPC-TP2-1
  • Availability: In Stock

  • $545.00

Tags: tyr p 2, ELISA 2.0, pre-coated plate, mold storage mite allergen,