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Gad m 1 (Cod Fish) ELISA 2.0 kit - One plate (EPC-GM1-1)

  • Gad m 1 (Cod Fish) ELISA 2.0 kit - One plate (EPC-GM1-1)

ELISA 2.0 kit for measuring Gadus morhua (Cod fish beta-parvalbumin) allergen, Gad m 1.
Antibodies: pAb/pAb
Standard Range: 50-0.10ng/mL
Limit of Detection: 0.39ng/mL
Background: OD<0.08ng/ml at 450nm
Coefficient of Determination: R-squared>0.98
Contents:

Plate: Pre-coated with anti-Gad m1 purified polyclonal antibody
Vial 1: (white top) Gad m 1 allergen standard
Concentration: 500ng/ml
Vial 2: (brown) biotinylated anti-Gad m 1 purified polyclonal antibody
Vial 3: (blue top) Streptavidin-Peroxidase
Bottle 1: Wash buffer, (10x concentrate)
Bottle 2: Assay buffer, (10x concentrate)
Bottle 3: TMB developing substrate
Bottle 4: Stop solution (0.5N sulfuric acid)
Storage: The ELISA 2.0 kit should be stored at 2-8°C.
Expiry: See product insert.
Not Provided:

Type I ultrapure water or 18.2MΩ de-ionized water
Volumetric measuring equipment (e.g. serological pipette, graduated cylinder)
Clean containers for buffer and reagent preparation
Calibrated single and multi-channel micropipettes and tips
Vortex mixer
Plate reader capable of reading absorbance at 450nm
Analysis software (recommended, but not required)
Notes: The allergen standard is recommended for immunoassay calibration purposes only.
A list of frequently asked questions and troubleshooting guide can be found under the ‘Support’ tab on our website.
Product Resources:

EPC-GM1-1 Certificate of Analysis

Validation Performance Data

For research and commercial use in vitro: not for human in vivo or therapeutic use.
  Certificate of Analysis
Pre-coated Plate: 96-well polystyrene microtiter plate coated with
monoclonal antibody and treated with stabilizing
agent. Sealed in foil pouch with desiccant.
Polyclonal Antibody: Purified rabbit polyclonal IgG
Immunogen: Natural Gad m 1
Isotype: Multiple
Specificity: Binds to an epitope on cod (beta-parvalbumin) allergen, Gadus morhua, Gad m 1.
Purification: Purified by affinity chromatography. Single heavy and light chain bands on SDS-PAGE.
  Certificate of Analysis
Detection Antibody: Purified rabbit polyclonal IgG
Immunogen: Natural Gad m 1
Isotype Multiple
Specificity: Binds to an epitope on cod (beta-parvalbumin) allergen, Gadus morhua, Gad m 1.
Purification: Purified by affinity chromatography. Single heavy and light chain bands on SDS-PAGE.
Activity: Biotinylated and titrated for use in ELISA at 1/1000 dilution. Prepared in 1% BSA/50% glycerol/PBS, pH 7.4, 0.22 μm filtered, preservative free.
  Certificate of Analysis
Allergen Standard: Natural Gad m 1 prepared in 1% BSA/50%
glycerol/PBS, pH 7.4.
Concentration/Calibration: 500 ng/mL (based on OD280)
References:  
 
 


 

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  • Product Code: EPC-GM1-1
  • Availability: In Stock

  • $595.00

Tags: Gad m 1, ELISA 2.0, pre-coated plate,