Amb a 1 ELISA 2.0 kit

ELISA 2.0 kit for measuring Ambrosia artemisiifolia allergen, Amb a 1, pollen

Antibodies: 2B6/4H7

Standard Range: 200-0.4 ng/mL

Limit of Detection: 0.78 ng/mL

Background: OD<0.08 at 450nm

Coefficient of Determination: R-squared>0.98

Contents:
Plates: Pre-coated with anti-Amb a 1 monoclonal antibody 2B6
Vial 1: (white top) Amb a 1 allergen standard
Concentration: 2000 ng/ml
Vial 2: (brown) anti-Amb a 1 antibody 4H7
Vial 3: (blue top) Streptavidin-Peroxidase
Bottle 1: Wash buffer, (10x concentrate)
Bottle 2: Assay buffer, (10x concentrate)
Bottle 3: TMB developing substrate
Bottle 4: Stop solution (0.5N sulfuric acid)

Storage: The ELISA 2.0 kit should be stored at 2-8°C.

Expiry: See product insert.

Not Provided:
Type I ultrapure water or 18.2MΩ de-ionized water
Volumetric measuring equipment (e.g. serological pipette, graduated cylinder)
Clean containers for buffer and reagent preparation
Calibrated single and multi-channel micropipettes and tips
Vortex mixer
Plate reader capable of reading absorbance at 450nm
Analysis software (recommended, but not required)

Notes: The allergen standard is recommended for immunoassay calibration purposes only.
A list of frequently asked questions and troubleshooting guide can be found under the ‘Support’ tab on our website.

Product Resources:
EPC-AM1-1 Certificate of Analysis
Validation Performance Data and Protocol

For research and commercial use in vitro: not for human in vivo or therapeutic use