Ana o 3 ELISA 2.0 kit
ELISA 2.0 kit for measuring Anacardium occidentale allergen, Ana o 3, cashew.
Antibodies: 1H4/4A11
Standard Range: 40-0.08 ng/mL
Limit of Detection: 0.16 ng/mL
Background: OD<0.08 at 450nm
Coefficient of Determination: R-squared>0.98
Contents:
Plates: Pre-coated with anti-Ana o 3 monoclonal antibody 1H4
Vial 1: (white top) Ana o 3 allergen standard
Concentration: 400 ng/ml
Vial 2: (brown) anti-Ana o 3 antibody 4A11
Vial 3: (blue top) Streptavidin-Peroxidase
Bottle 1: Wash buffer, (10x concentrate)
Bottle 2: Assay buffer, (10x concentrate)
Bottle 3: TMB developing substrate
Bottle 4: Stop solution (0.5N sulfuric acid)
Storage: The ELISA 2.0 kit should be stored at 2-8°C.
Expiry: See product insert.
Not Provided:
Type I ultrapure water or 18.2MΩ de-ionized water
Volumetric measuring equipment (e.g. serological pipette, graduated cylinder)
Clean containers for buffer and reagent preparation
Calibrated single and multi-channel micropipettes and tips
Vortex mixer
Plate reader capable of reading absorbance at 450nm
Analysis software (recommended, but not required)
Notes: The allergen standard is recommended for immunoassay calibration purposes only.
A list of frequently asked questions and troubleshooting guide can be found under the ‘Support’ tab on our website.
Product Resources:
EPC-AO3 Certificate of Analysis and Protocol
Validation Performance Data
For research and commercial use in vitro: not for human in vivo or therapeutic use.





