Asp f 1 ELISA 2.0 kit

ELISA 2.0 kit for measuring Aspergillus fumigatus allergen, Asp f 1, mold.

Antibodies: 4A6/pAb

Standard Range: 40-0.08ng/mL

Limit of Detection: 0.32ng/mL

Background: OD<0.08 at 450nm

Coefficient of Determination: R-squared>0.98

Contents: Plates: Pre-coated with anti-Asp f 1 monoclonal antibody 4A6
Vial 1: (white top) nAsp f 1 allergen standard
Concentration: 400ng/ml
Vial 2: (brown) Rabbit anti-Asp f 1
Vial 3: (blue top) Peroxidase conjugated Goat anti. Rabbit IgG
Bottle 1: Wash buffer, (10x concentrate)
Bottle 2: Assay buffer, (10x concentrate)
Bottle 3: TMB developing substrate
Bottle 4: Stop solution (0.5N sulfuric acid)

Storage: Store at 2-8°C.

Expiry: See product insert.

Not Provided: Type I ultrapure water or 18.2MΩ de-ionized water
Volumetric measuring equipment (e.g. serological pipette, graduated cylinder)
Clean containers for buffer and reagent preparation
Calibrated single and multi-channel micropipettes and tips
Vortex mixer
Plate reader capable of reading absorbance at 450nm
Analysis software (recommended, but not required)

Notes: The allergen standard is recommended for immunoassay calibration purposes only.
A list of frequently asked questions and troubleshooting guide can be found under the ‘Support’ tab on our website.

Product Resources:
EPC-AF1 Certificate of Analysis and Protocol
Validation Performance Data

For research and commercial use in vitro: not for human in vivo or therapeutic use.

References:

1. Arruda, LK, Platts-Mills, TAE, Fox, JW and Chapman, MD (1990). Aspergillus fumigatus allergen I, a major IgE binding protein, is a member of the mitogillin family of cytotoxins. J. Exp. Med., 172:1529-1532.
2. Arruda LK, Platts-Mills TAE, Longbottom JL, Chapman MD (1992). Aspergillus fumigatus: Identification of 16kd, 18kd and 45kd antigens recognized by human IgG and IgE antibodies and murine monoclonal antibodies. J. Allergy Clin. Immunol. 89:1166-1176.
3. Arruda LK, Mann BJ, Chapman MD (1992). Selective expression of a major allergen and cytotoxin, Asp f 1, in Aspergillus fumigatus. J. Immunol. 149:3354- 3359.
4. Sporik RB, Arruda LK, Woodfolk J, Chapman MD, Platts-Mills TAE (1993). Environmental exposure to Aspergillus fumigatus allergen 1 (Asp f 1). Clin. Exp. Allergy 23:326-331.
5. El-Dahr JM, Fink R, Selden R, Arruda LK, Platts-Mills TAE, Heymann PW (1994). Development of immune responses to Aspergillus at an early age in children with cystic fibrosis. Am. J. Respir. Crit. Care Med. 150:1513-1518.
6. Vailes LD, Sridhara S, Cromwell O, Weber B, Breitenbach M, Chapman MD (2001). Quantitation of the major fungal allergens, Alt a 1 and Asp f 1, in commercial allergenic products. J. Allergy Clin. Immunol. 107:641-646.
7. Chapman MD (2006). Challenges associated with indoor moulds: Health effects, immune response and exposure assessment. Medical Mycology. 44:S29-S32.