Bet v 1 ELISA 2.0 EP kit

ELISA 2.0EP kit for measuring Birch pollen allergen, Bet v 1.

Cross-validated against the Candidate Standard Method for the European Pharmacopoeia

Antibodies: 5B4/6H4

Standard Range: 100-0.19 ng/ml

Limit of Detection: 0.39ng/ml

Background: OD<0.08 at 450nm

Coefficient of Determination: R-squared>0.98

Contents: Plate: Pre-coated with anti-Bet v 1 monoclonal antibody 5B4
Vial 1: (white top) Bet v 1 allergen standard
Concentration: 1000 ng/ml
Vial 2: (brown) Biotinylated monoclonal antibody 6H4
Vial 3: (blue top) Streptavidin-peroxidase
Bottle 1: Wash buffer, (10x concentrate)
Bottle 2: Assay buffer, (10x concentrate)
Bottle 3: TMB developing substrate
Bottle 4: Stop solution (0.5N sulfuric acid)
Storage: Store at 2-8°C.

Expiry: See product insert.

Not Provided: Type I ultrapure water or 18.2MΩ de-ionized water
Volumetric measuring equipment (e.g. serological pipette, graduated cylinder)
Clean containers for buffer and reagent preparation
Calibrated single and multi-channel micropipettes and tips
Vortex mixer
Plate reader capable of reading absorbance at 450nm
Analysis software (recommended, but not required)

Notes: The allergen standard is recommended for immunoassay calibration
purposes only.
A list of frequently asked questions and troubleshooting guide can be found under the ‘Support’ tab on our website.

Product Resources:
EPC-BV1 Certificate of Analysis and Protocol
Validation Performance Data

For research and commercial use in vitro: not for human in vivo or therapeutic use.

References:

1. Kaul S, et al. Standardization of allergen products: 3. Validation of candidate European Pharmacopoeia standard methods for quantification of major birch allergen Bet v 1. Allergy. 2016; 71:1414.
2. Vieths S, et al. Establishment of recombinant major allergens Bet v 1 and Phl p 5a as Ph. Eur. Reference standards and validation of ELISA methods for their measurement. Results from feasibility studies. Pharmeur Bio Sci Notes. 2012; 2012:118.
3. Himly M, et al. Standardization of allergen products: 1. Detailed characterization of GMP-produced recombinant Bet v 1.0101 as biological reference preparation. Allergy. 2009; 64:1038.