Blo t 5 ELISA 2.0 kit

ELISA 2.0 kit for measuring Blomia tropicalis allergen, Blo t 5.

Antibodies: pAb/pAb

Standard Range: 30-0.06ng/mL

Limit of Detection: 0.12ng/mL

Background: OD<0.08 at 450nm

Coefficient of Determination: R-squared>0.98

Contents:
Plate: Pre-coated with anti-Blo t 5 purified polyclonal antibody
Vial 1: (white top) Blo t 5 allergen standard
Concentration: 300ng/ml
Vial 2: (brown) Biotinylated purified polyclonal antibody
Vial 3: (blue top) Streptavidin-peroxidase
Bottle 1: Wash buffer, (10x concentrate)
Bottle 2: Assay buffer, (10x concentrate)
Bottle 3: TMB developing substrate
Bottle 4: Stop solution (0.5N sulfuric acid)

Storage: Store at 2-8°C.

Expiry: See product insert.

Not Provided:
Type I ultrapure water or 18.2MΩ de-ionized water
Volumetric measuring equipment (e.g. serological pipette, graduated cylinder)
Clean containers for buffer and reagent preparation
Calibrated single and multi-channel micropipettes and tips
Vortex mixer
Plate reader capable of reading absorbance at 450nm
Analysis software (recommended, but not required)

Notes: The allergen standard is recommended for immunoassay calibration purposes only.
A list of frequently asked questions and troubleshooting guide can be found under the ‘Support’ tab on our website.

Product Resources:
EPC-BT5 Certificate of Analysis and Protocol
Validation Performance Data

For research and commercial use in vitro: not for human in vivo or therapeutic use.

References:

1. Arruda LK, Rizzo MC, Chapman MD, Fernandez-Caldas E, Baggio D, Platts-Mills TAE, Naspitz CK. Exposure and sensitization to dust mite allergens among asthmatic children in Sno Paulo, Brazil. Clinical Exp Allergy 1991; 21:433-439.
2. Vailes LD, Rizzo MC, Naspitz CK, Montealegre F, Fernandez-Caldas E, Platts-Mills TAE, Chapman MD, Arruda LK. Analysis of IgE antibody responses to D. pteronyssinus and Blomia tropicalis using recombinant group 5 allergens. J Allergy Clin Immunol 1996; 97:213 (Abstract).
3. Arruda LK, Vailes LD, Platts-Mills TAE, Fernandez-Caldas E, Montealegre F, Lin KL, Chua KY, Rizzo MC, Naspitz CK, Chapman MD. Sensitization to Blomia tropicalis in patients with asthma and identification of allergen Blo t 5. Amer J Resp Crit Care Med 1997; 155:343-350.