Bos d 11 ELISA 2.0 kit

ELISA 2.0 kit for measuring Bos domesticus β-casein allergen, Bos d 11, milk.

Antibodies: CC11/VBIC

Standard Range: 1,000-1.95ng/mL

Limit of Detection: 7.81ng/mL

Background: OD<0.08 at 450nm

Coefficient of Determination: R-squared>0.98

Contents:
Plate: Pre-coated with anti-Bos d 11 monoclonal antibody CC11
Vial 1: (white top) Bos d 11 allergen standard
Concentration: 10,000ng/ml
Vial 2: (brown) Biotinylated monoclonal antibody VBIC
Vial 3: (blue top) Streptavidin-peroxidase
Bottle 1: Wash buffer, (10x concentrate)
Bottle 2: Assay buffer, (10x concentrate)
Bottle 3: TMB developing substrate
Bottle 4: Stop solution (0.5N sulfuric acid)

Storage: Store at 2-8°C

Expiry: See product insert.

Not Provided:
Type I ultrapure water or 18.2MΩ de-ionized water
Volumetric measuring equipment (e.g. serological pipette, graduated cylinder)
Clean containers for buffer and reagent preparation
Calibrated single and multi-channel micropipettes and tips
Vortex mixer
Plate reader capable of reading absorbance at 450nm
Analysis software (recommended, but not required)

Notes: The allergen standard is recommended for immunoassay calibration purposes only.
A list of frequently asked questions and troubleshooting guide can be found under the ‘Support’ tab on our website.

Product Resources:
EPC-BD11 Certificate of Analysis and Protocol
Validation Performance Data

For research and commercial use in vitro: not for human in vivo or therapeutic use.

References:

1. Luyt D, Ball H, Makwana N, Green MR, Bravin K, et al. BSACI Guideline for the Diagnosis and Management of Cow's Milk Allergy. Clinical and Experimental Allergy: Journal of the British Society for Allergy and Clinical Immunology. 2014.
2. Fiocchi A, Bouygue GR, Albarini M, Restani P. Molecular diagnosis of cow’s milk allergy. Curr Opin Allergy Clin Immunol. 2011 Jun;11(3):216-21.