Der p 23 ELISA 2.0 kit

ELISA 2.0 kit for measuring D. pteronyssinus allergen, Der p 23, house dust mite.

Antibodies: pAb/7A8

Standard Range: 50-0.1ng/mL

Limit of Detection: 0.39ng/mL

Background: OD<0.08 at 450nm

Coefficient of Determination: R-squared>0.98

Contents:
Plate: Pre-coated with purified rabbit polyclonal anti Der p 23 antibody
Vial 1: (white top) Der p 23 allergen standard
Concentration: 500ng/ml
Vial 2: (brown) Biotinylated monoclonal antibody 7A8
Vial 3: (blue top) Streptavidin-peroxidase
Bottle 1: Wash buffer, (10x concentrate)
Bottle 2: Assay buffer, (10x concentrate)
Bottle 3: TMB developing substrate
Bottle 4: Stop solution (0.5N sulfuric acid)

Storage: Store at 2-8°C.

Expiry: See product insert.

Not Provided:
Type I ultrapure water or 18.2MΩ de-ionized water
Volumetric measuring equipment (e.g. serological pipette, graduated cylinder)
Clean containers for buffer and reagent preparation
Calibrated single and multi-channel micropipettes and tips
Vortex mixer
Plate reader capable of reading absorbance at 450nm
Analysis software (recommended, but not required)

Notes: The allergen standard is recommended for immunoassay calibration purposes only.
A list of frequently asked questions and troubleshooting guide can be found under the ‘Support’ tab on our website.

Product Resources:
EPC-DP23 Certificate of Analysis and Protocol
Validation Performance Data

For research and commercial use in vitro: not for human in vivo or therapeutic use.

License Agreement: InBio has executed a patent license agreement with Biomay AG (Vienna, Austria), which covers commercial sale of kits for measurement of Der p 23 by immunoassay.

References:

1. Weghofer M et al. Identification of Der p 23, a peritrophin-like protein, as a new major Dermatophagoides pteronyssinus allergen associated with the peritrophic matrix of mite fecal pellets. J Immunol 2013; 190: 3059-67.
2. Mueller GA, et al. Serological, genomic and structural analyses of the major mite allergen Der p 23. Clin Exp Allergy 2016. 46(2): 365-76.
3.  Posa D, Perna S, Resch Y et al. Evolution and predictive value of IgE responses toward a comprehensive panel of house dust mite allergens during the first 2 decades of life. J Allergy CLin Immunol 2017; 139: 541-9.
4. Zidarn M, Robic M, Krivec A et al. Clinical and immunological differences between asymptomatic HDM-sensitized and HDM-allergic rhinitis patients. Clin Exp Allergy 2019; 49: 808-18.