Gly m 5 ELISA 2.0 kit

ELISA 2.0 kit for measuring Glycine max (beta-conglycinin) allergen, Gly m 5, soy.

Antibodies: 6F6/1B9

Standard Range: 500-0.98 ng/ml

Limit of Detection: 1.95ng/ml

Background: OD<0.08 at 450nm

Coefficient of Determination: R-squared>0.98

Contents: Plate: Pre-coated with anti-Gly m 5 monoclonal antibody 6F6
Vial 1: (white top) Gly m 5 allergen standard
Concentration: 5000 ng/ml
Vial 2: (brown) Biotinylated monoclonal antibody 1B9
Vial 3: (blue top) Streptavidin-peroxidase
Bottle 1: Wash buffer, (10x concentrate)
Bottle 2: Assay buffer, (10x concentrate)
Bottle 3: TMB developing substrate
Bottle 4: Stop solution (0.5N sulfuric acid)

Storage: Store at 2-8°C.

Expiry: See product insert.

Not Provided: Type I ultrapure water or 18.2MΩ de-ionized water
Volumetric measuring equipment (e.g. serological pipette, graduated cylinder)
Clean containers for buffer and reagent preparation
Calibrated single and multi-channel micropipettes and tips
Vortex mixer
Plate reader capable of reading absorbance at 450nm
Analysis software (recommended, but not required)

Notes: The allergen standard is recommended for immunoassay calibration
purposes only.
A list of frequently asked questions and troubleshooting guide can be found under the ‘Support’ tab on our website.

Product Resources:
EPC-GM5 Certificate of Analysis and Protocol
Validation Performance Data

For research and commercial use in vitro: not for human in vivo or therapeutic use.