Mus m 1 ELISA 2.0 kit

ELISA 2.0 kit for measuring Mouse Urinary Protein (MUP) allergen, Mus m 1.

Antibodies: pAb/pAb

Standard Range: 25-0.05 ng/mL

Limit of Detection: 0.19ng/mL

Background: OD<0.08 at 450nm

Coefficient of Determination: R-squared>0.98

Contents:
Plate: Pre-coated with anti-Mus m 1 polyclonal rabbit capture antibody.
Vial 1: (white top) Mus m 1 allergen standard
Concentration: 250 ng/mL
Vial 2: (brown) Biotinylated polyclonal rabbit anti-Mus m 1 antibody.
Vial 3: (blue top) Streptavidin-peroxidase
Bottle 1: Wash buffer, (10x concentrate)
Bottle 2: Assay buffer, (10x concentrate)
Bottle 3: TMB developing substrate
Bottle 4: Stop solution (0.5N sulfuric acid)

Storage: Store at 2-8°C

Expiry: See product insert.

Not Provided:
Type I ultrapure water or 18.2MΩ de-ionized water
Volumetric measuring equipment (e.g. serological pipette, graduated cylinder)
Clean containers for buffer and reagent preparation
Calibrated single and multi-channel micropipettes and tips
Vortex mixer
Plate reader capable of reading absorbance at 450nm
Analysis software (recommended, but not required)

Notes: The allergen standard is recommended for immunoassay calibration purposes only.
A list of frequently asked questions and troubleshooting guide can be found under the ‘Support’ tab on our website.

Product Resources:
EPC-MM1 Certificate of Analysis and Protocol
Validation Performance Data

For research and commercial use in vitro: not for human in vivo or therapeutic use.

References:

1. Phipatanakul W, Eggleston PA, Wright EC, Wood RA. Mouse allergen. I. The prevalence of mouse allergen in inner-city homes. The National Cooperative Inner-City Asthma Study. J Allergy Clin Immunol. 2000;106:1070-4.
2. Phipatanakul W, Eggleston PA, Wright EC, Wood RA. Mouse allergen. II. The relationship of mouse allergen exposure to mouse sensitization and asthma morbidity in inner-city children with asthma. J Allergy Clin Immunol. 2000;106:1075-80.
3. Permaul P, Hoffman E, Fu C, Sheehan W, Baxi S, Gaffin J, Lane J, Bailey A, King E, Chapman M, Gold D, Phipatanakul W. Allergens in urban schools and homes of children with asthma. Pediatr Allergy Immunol. 2012;23:543-9.
4. Ahluwalia SK, Peng RD, Breysse PN, Diette GB, Curtin-Brosnan J, Aloe C, Matsui EC. Mouse allergen is the major allergen of public health relevance in Baltimore City. J Allergy Clin Immunol. 2013;132:830-5.
5. Ownby DR. Will the real inner-city allergen please stand up? J Allergy Clin Immunol. 2013;132:836-7.