Natural Der p 1 (NA-DP1-1)

Allergen: nDer p 1 (Dermatophagoides pteronyssinus allergen 1), house dust mite

Unit: 250µg

Source: D.pteronyssinus culture medium

Mol. Wt: 24 kD

Purification: From mite culture by affinity chromatography. Purity on silver stained SDS-PAGE >95%

Concentration: See package insert.

Formulation: Preservative-free and carrier-free in phosphate buffered saline, pH 7.4. Sterile filtered.

Storage: Store at -20ºC

Notes: (1) Not tested for endotoxin. (2) Similar nDer p 1 preparations have been used in studies to analyze the cysteine protease activity of Der p 1, for T cell proliferation studies and antibody binding.

PDB: 1XKG, 2AS8, 3F5V X-ray crystal structures

Product Resources: Natural Der p 1 Certificate of Analysis

Allergens are provided for research and commercial use in vitro: not for human in vivo or therapeutic use.

References:

1. Lombardero M, Heymann PW, Platts-Mills TAE, Fox JW, Chapman MD. Conformational stability of B cell epitopes on Group 1 and Group II Dermatophagoides spp. Allergens. Effect of thermal and chemical denaturation on the binding of murine IgG and human IgE antibodies. J Immunol 1990;144:1353-1360.
2. Hewitt CRA, Brown AP, Hart BJ, Pritchard DL. A major house dust mite allergen disrupts the Immunoglobulin E network by selectively cleaving CD23: innate protection by antiproteases. J Exp Med 1995;182:1537-1544.
3. Schultz O, Laing P, Sewell HF, Shakib F. Der p 1, a major allergen of the house dust mite, proteolytically cleaves the low affinity receptor for human IgE (CD23). Eur J Immunol 1995;25:3191-3194.
4. King C, Brennan S, Thompson PJ, Stewart GA. Dust mite proteolytic allergens induce cytokine release from cultured airway epithelium. J Immunol 1998; 161:3645-3651.
5. Gough L, Schultz O, Sewell HF, Shakib F. The cysteine protease activity of the major house dust mite allergen Der p 1 selectively enhances the immunoglobulin E antibody responses. J Exp Med 1999; 190:1897-1901.
6. Wan H, Winton HL, Soeller C, Tovey ER, Gruenert DC, Thompson PJ, Stewart GA, Taylor GW, Garrod DR, Cannell MD, Robinson C. Der p 1 facilitates transepithelial allergen delivery by disruption of tight junctions. J Clin Invest 1999; 104:123-133.
7. Chruszcz M, Chapman MD, Vailes LD, Stura EA, Saint-Remy JM, Minor W, Pomés A. Crystal structures of mite allergens Der f 1 and Der p 1 reveal differences in surface-exposed residues that may influence antibody binding. J Mol Biol, 2009;386:520-530.
8. Chruszcz M, Pomés A, Glesner J, Vailes LD, Osinski T, Porebski PJ, Majorek KA, Heymann PW, Platts-Mills TAE, Minor W, Chapman MD. Molecular determinants for antibody binding on Group 1 house dust mite allergens. J Biol Chem 2012; 287:7388-7398.