Natural Der p 2 (NA-DP2-1)

Allergen: nDer p 2 (Dermatophagoides pteronyssinus allergen 2), house dust mite

Unit: 250µg

Source: D.pteronyssinus culture medium

Mol. Wt: 14 kD

Purification: From mite culture by affinity chromatography using anti-mite Group 2 monoclonal antibody 7A1. Purity >95% by SDS-PAGE.

Concentration: See package insert.

Formulation: Preservative-free and carrier-free in phosphate buffered saline, pH 7.4. Filtered through 0.22µ filter.

Storage: Store at -20ºC

Notes: (1) The preparation shows a major Der p 2 band at 14kD on silver stained SDS-PAGE having purity > 95%.
(2) Not tested for endotoxin.

PDB: 1KTJ X-ray crystal structure; 1A9V NMR structure

Product Resources: Natural Der p 2 Certificate of Analysis

Allergens are provided for research and commercial use in vitro: not for human in vivo or therapeutic use.

References:

1. Heyman PW, Chapman MD, Fox J, Aalberse, RC, PlattsMills, TAE. Antigenic and structural analysis of the Group II allergens (Derp II and Der f II) from house dust mites (Dermatophagoides sp). J Allergy Clin Immunology 1989;83:1055-67.
2. Mueller GA, Smith AM, Williams DC Jr., Hakkaart GAJ, Aalberse RC, Chapman MD, Rule, GS, Benjmin DC. Expression and secondary structure determination by NMR methods of the major house dust mite allergen Der p 2. J Biol Chem 1997;272:26893-8.
3. Smith AM, Benjamin DC, Hozic N, Derewenda U, Smith WA, Thomas WR, Gafvelin G, van Hage-Hamsten M, Chapman MD. The molecular basis of antigenic cross-reactivity between the group 2 mite allergens. J Allergy Clin Immunol 2001;107:977-84.
4. Trompette A, Divanovic S, Visintin A, Blanchard C, Hegde RS, Madan R, Thorne PS, Wills-Karp M, Gioannini TL, Weiss JP, Karp CL. Allergenicity resulting from functional mimicry of a Toll-like receptor complex protein. Nature 2008, Dec. 7.