Per a 7 ELISA 2.0 kit
ELISA 2.0 kit for measuring Periplaneta americana (tropomyosin) allergen, Per a 7, cockroach.
Antibodies: pAb/1A6
Standard Range: 50.0 - 0.1 ng/ml
Limit of Detection: 0.19ng/ml
Background: OD<0.08 at 450nm
Coefficient of Determination: R-squared>0.98
Contents:
Plate: Pre-coated with anti-shrimp tropomyosin polyclonal antibody
Vial 1: (white top) Per a 7 allergen standard
Concentration: 500 ng/ml
Vial 2: (brown) Biotinylated monoclonal antibody 1A6
Vial 3: (blue top) Streptavidin-peroxidase
Bottle 1: Wash buffer, (10x concentrate)
Bottle 2: Assay buffer, (10x concentrate)
Bottle 3: TMB developing substrate
Bottle 4: Stop solution (0.5N sulfuric acid)
Storage: Store at 2-8°C.
Expiry: See product insert.
Not Provided: Type I ultrapure water or 18.2MΩ de-ionized water
Volumetric measuring equipment (e.g. serological pipette, graduated cylinder)
Clean containers for buffer and reagent preparation
Calibrated single and multi-channel micropipettes and tips
Vortex mixer
Plate reader capable of reading absorbance at 450nm
Analysis software (recommended, but not required)
Notes: The allergen standard is recommended for immunoassay calibration purposes only.
A list of frequently asked questions and troubleshooting guide can be found under the ‘Support’ tab on our website.
Product Resources:
EPC-PA7 Certificate of Analysis and Protocol
Validation Performance Data
For research and commercial use in vitro: not for human in vivo or therapeutic use.



