Phl p 5 ELISA 2.0 kit

ELISA 2.0 kit for measuring Timothy grass pollen allergen, Phl p 5.

Antibodies: 1D11/Bo1

Standard Range: 250-0.49 ng/ml

Limit of Detection: 0.98ng/ml

Background: OD<0.08 at 450nm

Coefficient of Determination: R-squared>0.98

Contents:
Plate: Pre-coated with anti-Phl p 5 monoclonal antibody 1D11
Vial 1: (white top) Phl p 5a allergen standard
Concentration: 2500 ng/ml
Vial 2: (brown) Biotinylated monoclonal antibody Bo1
Vial 3: (blue top) Streptavidin-peroxidase
Bottle 1: Wash buffer, (10x concentrate)
Bottle 2: Assay buffer, (10x concentrate)
Bottle 3: TMB developing substrate
Bottle 4: Stop solution (0.5N sulfuric acid)

Storage: Store at 2-8°C.

Expiry: See product insert.

Not Provided: Type I ultrapure water or 18.2MΩ de-ionized water
Volumetric measuring equipment (e.g. serological pipette, graduated cylinder)
Clean containers for buffer and reagent preparation
Calibrated single and multi-channel micropipettes and tips
Vortex mixer
Plate reader capable of reading absorbance at 450nm
Analysis software (recommended, but not required)

Notes: The allergen standard is recommended for immunoassay calibration purposes only.
A list of frequently asked questions and troubleshooting guide can be found under the ‘Support’ tab on our website.

Product Resources:
EPC-PP5 Certificate of Analysis and Protocol
Validation Performance Data

For research and commercial use in vitro: not for human in vivo or therapeutic use.

References:

1. Müller WD, Diener C, Jung K, Jäger L. Antigens of Timothy and other grass pollen extracts identified by monoclonal antibodies. Allergol Immunopathol (Madr). 1988;16:315-20.
2. Fahlbusch B, Müller WD, Diener CH, Jäger L. Detection of crossreactive determinants in grass pollen extracts using monoclonal antibodies against group IV and group V allergens. Clin Exp Allergy. 1993;23:51-60.
3. Petersen A, Becker WM, Schlaak M. Epitope analysis of isoforms of the major allergen Phl p V by fingerprinting and microsequencing. Clin Exp Allergy. 1994;24:250-6.
4. S. Vieths, D. Barber, M. Chapman, A. Costanzo, A. Daas, H. Fiebig, K.M. Hanschmann, M. Hrabina, S. Kaul, A. Ledesma, P. Moingeon, G. Reese, C. Schörner, R. van Ree, B.
5. Weber, K.H. Buchheit. Establishment of recombinant major allergens Bet v 1 and Phl p 5a as Ph. Eur. Reference standards and validation of ELISA methods for their measurement. Pharmeuropa Bio&SN October 2012; 118-13.