Protein Identification (IBMS-ID)

Description: Identification of proteins in a gel band/spot or in solution.

Gel bands/Gel spots. We accept any type of gel matrix and most stains (please avoid the Bio-Rad Silver Stain Plus Kit). Make sure to cut the band close to your target, the gel band dimensions should be ~5mm x 2mm.
Protein in solution. Required protein concentration of ≥1µg/µL in 50-100µL of buffer. Please provide detailed buffer information with your sample submission, as well as sequence info on the target protein(s).

Sample preparation: Digestion with trypsin, Reduction & Alkylation

LC-MS/MS: Digested samples are analyzed with LC-MS/MS

Data analysis: Protein identification performed with Sequest HT

Report generation: PDF/Excel

Turnaround time: 2 weeks

Sample Submission Form