Rat n 1 ELISA 2.0 kit

ELISA 2.0 kit for measuring Rat Urinary Protein (RUP) allergen, Rat n 1.

Antibodies: RUP-6/RUP-1

Standard Range: 50-0.1 ng/mL

Limit of Detection: 0.39ng/mL

Background: OD<0.08 at 450nm

Coefficient of Determination: R-squared>0.98

Contents:
Plate: Pre-coated with anti-Rat n 1 monoclonal antibody RUP-6
Vial 1: (white top) Rat n 1 allergen standard
Concentration: 500 ng/ml
Vial 2: (brown) Biotinylated monoclonal antibody RUP-1
Vial 3: (blue top) Streptavidin-peroxidase
Bottle 1: Wash buffer, (10x concentrate)
Bottle 2: Assay buffer, (10x concentrate)
Bottle 3: TMB developing substrate
Bottle 4: Stop solution (0.5N sulfuric acid)

Storage: Store at 2-8°C

Expiry: See product insert.

Not Provided:
Type I ultrapure water or 18.2MΩ de-ionized water
Volumetric measuring equipment (e.g. serological pipette, graduated cylinder)
Clean containers for buffer and reagent preparation
Calibrated single and multi-channel micropipettes and tips
Vortex mixer
Plate reader capable of reading absorbance at 450nm
Analysis software (recommended, but not required)

Notes: The allergen standard is recommended for immunoassay calibration
purposes only.
A list of frequently asked questions and troubleshooting guide can be found under the ‘Support’ tab on our website.

Product Resources:
EPC-RN1 Certificate of Analysis and Protocol
Validation Performance Data

For research and commercial use in vitro: not for human in vivo or therapeutic use.

References:

1. Renstrom A, Larsson PH, Malmberg P, Bayard C. A new amplified monoclonal rat allergen assay used for evaluation of ventilation improvements in animal rooms. J Allergy Clin Immunol. 1997 Nov;100(5):649-55.
2. Renstrom A, Gordon S, Larsson PH, Tee RD, Newman Taylor AJ, Malmberg P. Comparison of a radioallergosorbent (RAST) inhibition method and a monoclonal enzyme linked immunosorbent assay (ELISA) for aeroallergen measurement. Clin Exp Allergy. 1997 Nov;27(11):1314-21.