Recombinant Bet v 1 (RP-BV1-1)

Allergen: rBet v 1 (Betula pendula allergen 1.0101), silver birch

Unit: 250µg

Source: Pichia pastoris

Mol. Wt: 18kD

Purification: Purified from Pichia pastoris culture by HPLC gel filtration. Purity >95 % by silver stained SDS-PAGE

Concentration: See product insert.

Formulation: Preservative-free and carrier-free in phosphate buffered saline, pH 7.4. Steriled filtered.

Storage: Store at -20ºC.

Notes: Glycosylation site N82 mutated (N82Q). Avoid repeated freeze-thaw cycles

Product Resources: Recombinant Bet v 1 Certificate of Analysis

Allergens are provided for research and commercial use in vitro: not for human in vivo or therapeutic use.

References:

1. Batard, T. et al: Characterization of wild-type recombinant Bet v 1a as a candidate vaccine against birch pollen allergy. Int Arch Allergy Immunol 2005, 136 (3), 239-249.
2. Breiteneder, H.et al: The gene coding for the major birch pollen allergen Bet v 1, is highly homologous to a pea disease resistance response gene. EMBO J 1989, 8 (7), 1935-1938.
3. Spangfort, M. D.et al: Dominating IgE-binding epitope of Bet v 1, the major allergen of birch pollen, characterized by X-ray crystallography and site-directed mutagenesis. J Immunol 2003, 171 (6), 3084-3090.
4. Swoboda, I.et al: Isoforms of Bet v 1, the major birch pollen allergen, analyzed by liquid chromatography, mass spectrometry, and cDNA cloning. J Biol Chem 1995, 270 (6), 2607-2613.
5. S. Vieths et al.: Establishment of recombinant major allergens Bet v 1 and Phl p 5a as Ph. Eur. Reference standards and validation of ELISA methods for their measurement. Pharmeuropa Bio & SN 2012; 118-13.