Recombinant Bla g 2 (RP-BG2-1)

Allergen: Bla g 2 (Blattella germanica allergen 2.0101)

Common name: German cockroach

Unit: 250µg

Source: Pichia pastoris (clone NIG rBla g 2, glycosylation site N93Q mutated)

Mol. Wt: ~37 kD

Purification: Purified from Pichia pastoris culture supernatant by monoclonal antibody affinity chromatography. Purity by silver stained SDS-PAGE > 95%.

Concentration: See product insert.

Formulation: Preservative and carrier-free in phosphate buffered saline, pH 7.4. Filtered through 0.22 µm filter.

Storage: Store at -20◦C. Re-freeze unused portion and aliquot into smaller quantities to avoid freeze/thaw cycles. Gently defrost at room temperature.

Notes: (1) One glycosylation site was removed in this recombinant leaving two glycosylation sites. The recombinant is glycosylated and has a slightly higher MW than natural. Bla g 2 (36kD). (2) This recombinant form was used to determine the crystal structure of Bla g 2.

Genbank: U28863 Mutation N93Q, substituted cag (Q) for aac (N)

PDB: 1YG9, 2NR6 (with Fab-7C11), X-ray crystal structures

Product Resources: Recombinant Bla g 2 Certificate of Analysis

Allergens are provided for research and commercial use in vitro:  not for human in vivo or therapeutic use.