Recombinant Der f 1 (RP-DF1-1)

Allergen: rDer f 1 (Dermatophagoides farinae allergen 1), house dust mite

Unit: 250µg

Source: Pichia pastoris

Mol. Wt: ~36-40kD

Purification: Purified from Pichia pastoris culture by mAb affinity chromatography. Purity >95% by SDS-PAGE.

Concentration: See product insert.

Formulation: Preservative-free and carrier-free in phosphate buffered saline, pH 7.4. Filtered through 0.22µm filter.

Storage: Store at -20ºC

Notes: (1) rDer f 1 produced in P. pastoris is glycosylated and has a higher MW than the natural allergen. The doublet is due to dimer formation.

Genbank: 3D6S X-ray crystal structure

Product Resources: Recombinant Der f 1 Certificate of Analysis

Allergens are provided for research and commercial use in vitro:  not for human in vivo or therapeutic use.

References:

1. Best EA, Stedman KE, Bozic CM, Hunter SW, Vailes LD, Chapman MD, McCall CA, McDermott MJ. A recombinant group 1 house dust mite allergen. rDer f 1, with biological activities similar to those of the native allergen. Prot Exp Pur 2000; 20:462-471.
2. Chapman MD, Smith AM, Vailes LD, Arruda LK, Dhanaraj V, Pomés A. Recombinant allergens for diagnosis and therapy of allergic disease. J Allergy Clin Immunol 2000; 106:409-418.
3. Chruszcz M, Chapman MD, Vailes LD, Stura EA, Saint-Remy JM, Minor W, Pomés A. Crystal structures of mite allergens Der f 1 and Der p 1 reveal differences in surface-exposed residues that may influence antibody binding. J Mol Biol, 2009;386:520-530.