Recombinant Der p 1 deglycosylated (RP-DP1D-1)

Allergen: Der p 1 (Dermatophagoides pteronyssinus allergen 1) deglycosylate

Common name: House dust mite

Unit: 250µg

Source: Pichia pastoris (clone N52Q, N-glycosylation site N52 mutated)

Mol. Wt: 24 kD

Purification: Purified from Pichia pastoris culture by multi-step column chromatography. Purity on silver stained SDS-PAGE >95%.

Concentration: See product insert.

Formulation: Preservative and carrier-free in sterile, 0.1M CAPS Buffer, pH 9.5. Filtered through 0.22 µm filter.

Storage: Store at -20◦C. Re-freeze unused portion and aliquot into smaller quantities to avoid freeze/thaw cycles. Gently defrost at room temperature.

Notes: (1) A LoTox™ product: Endotoxin ≤ 0.03 EU/µg. (2) Mature form of rDer p 1; proregion removed with acid dialysis. (3) Cysteine protease activity (DTT dependent): (see product insert) RFU @ 0.25µg/ml. (4) For stability purposes the product is shipped in CAPS Buffer pH9.5. It may be dialysed or diluted into a physiological buffer for cell culture purposes, but long-term storage in PBS may cause precipitation.

Genbank: U11695 = pro-Der p 1.0102 (difference is that in pro Der p 1.0105, V124 becomes A124)

PDB: 1XKG, 2AS8, 3F5V X-ray crystal structures

Product Resources: Recombinant Der p 1 DG Certificate of Analysis

Allergens are provided for research and commercial use in vitro:  not for human in vivo or therapeutic use.