Shrimp Tropomyosin ELISA 2.0 kit

ELISA 2.0 kit for measuring shrimp tropomyosin allergen.

Antibodies: 1A6/pAb

Standard Range: 50-0.1 ng/mL

Limit of Detection: 0.2ng/mL

Background: OD<0.08 at 450nm

Coefficient of Determination: R-squared>0.98

Contents:
Plate: Pre-coated with anti-Tropomyosin monoclonal antibody 1A6
Vial 1: (white top) Shrimp Tropomyosin allergen standard
Concentration: 500 ng/ml
Vial 2: (brown) Rabbit anti Shrimp Tropomyosin antibody
Vial 3: (blue top) Peroxidase-conjugated Goat Anti-Rabbit
Bottle 1: Wash buffer, (10x concentrate)
Bottle 2: Assay buffer, (10x concentrate)
Bottle 3: TMB developing substrate
Bottle 4: Stop solution (0.5N sulfuric acid)

Storage: The ELISA 2.0 kit should be stored at 2-8°C.

Expiry: See product insert.

Not Provided:
Type I ultrapure water or 18.2MΩ de-ionized water
Volumetric measuring equipment (e.g. serological pipette, graduated cylinder)
Clean containers for buffer and reagent preparation
Calibrated single and multi-channel micropipettes and tips
Vortex mixer
Plate reader capable of reading absorbance at 450nm
Analysis software (recommended, but not required)

Notes: The allergen standard is recommended for immunoassay calibration purposes only.
A list of frequently asked questions and troubleshooting guide can be found under the ‘Support’ tab on our website.

Product Resources:
EPC-TPM Certificate of Analysis and Protocol
Validation Performance Data

For research and commercial use in vitro: not for human in vivo or therapeutic use.

References:

1. Shanti KN, Martin BM, Nagpal S, Metcalfe DD, Subba Rao PV. Identification of tropomyosin as the major shrimp allergen and characterization of its IgE-binding epitopes. J Immunol. 1993; 151 (10):5354-5363.
2. Daul CB, Slattery M, Reese G, Lehrer SB. Identification of the major brown shrimp (Penaeus aztecus) allergen as the muscle protein tropomyosin. Int Arch Allergy Immunol. 1994; 105(1):49-55.
3. Jeoung BJ, Reese G, Hauck P, Oliver JB, Daul CB, Lehrer SB. Quantification of the major brown shrimp allergen Pen a 1 (tropomyosin) by a monoclonal antibody-based sandwich ELISA. J Allergy Clin Immunol. 1997; 100(2):229-234.
4. Santos AB, Rocha GM, Oliver C, Ferriani VPL, Lima RC, Palma MS, Sales VSF, Aalberse RC, Chapman MD, Arruda LK. Crossreactive IgE antibody responses to tropomyosins from Ascaris lumbricoides and cockroach. J Allergy Clin Immunol 2008; 121 (4):1040-1046.