Natural Der f 1 (NA-DF1-1)

Allergen:	nDer f 1 (Dermatophagoides farinae allergen 1),house dust mite
Unit:	250µg
Source:	D. farinae culture medium
Mol. Wt:	24kD
Purification:	From mite culture by affinity chromatography. Purity on silver stained SDS-PAGE >95%.
Concentration:	See package insert.
Formulation:	Preservative-free and carrier-free in phosphate buffered saline, pH 7.4. Sterile filtered.
Storage:	Store at -20ºC
Notes:	(1) Not tested for endotoxin
PDB:	3D6S X-ray crystal structure
Product Resources:	Natural Der f 1 Certificate of Analysis
Allergens are provided for research and commercial use in vitro: not for human in vivo or therapeutic use.
References:
Lombardero M, Heymann PW, Platts-Mills TAE, Fox JW, Chapman MD. Conformational stability of B cell epitopes on Group I and Group II Dermatophagoides spp. allergens. Effect of thermal and chemical denaturation on the binding of murine IgG and human IgE antibodies. J Immunol 1990; 144:1353-1360. Chruszcz M, Chapman MD, Vailes LD, Stura EA, Saint-Remy JM, Minor W, Pomés A. Crystal structures of mite allergens Der f 1 and Der p 1 reveal differences in surface- exposed residues that may influence antibody binding. J Mol Biol. 2009; 386:520-530. Chruszcz M, Pomés A, Glesner J, Vailes LD, Osinski T, Porebski PJ, Majorek KA, Heymann PW, Platts-Mills TAE, Minor W, Chapman MD. Molecular determinants for antibody binding on Group 1 house dust mite allergens. J Biol Chem 2012; 287:7388-7398.