Allergen: rAra h 2.0201 (Arachis hypogaea allergen 2), peanut Glycosylation site N127 mutated (N127Q

Unit: 250µg

Source: Pichia pastoris

Mol. Wt: 17-19 kD doublet

Purification: Purified from Pichia pastoris culture by multi-step HPLC. Purity > 95 % by silver stained SDS-PAGE

Concentration: See product insert.

Formulation: Preservative-free and carrier-free in phosphate buffered saline, 360mM NaCl, pH 7.4. Sterile filtered.

Storage: Store at -20ºC.

Notes: (1) rAra h 2 has a C-terminal 6xHis-tag.
(2) rAra h 2 appears as a doublet.
(3) Avoid repeated Freeze/Thaw cycles.

Product Resources: Recombinant Ara h 2 Certificate of Analysis

Allergens are provided for research and commercial use in vitro: not for human in vivo or therapeutic use.

References:

Burks AW, Williams LW, Connaughton C, Cockrell G, O’Brien TJ, Helm RM. Identification and characterization of a second major peanut allergen, Ara h II, with use of the sera of patients with atopic dermatitis and positive peanut challenge. J Allergy Clin Immunol 1992;90:962-9.
Sen M, Kopper R, Pons L, Abraham EC, Burks W, Bannon GA. Protein structure plays a critical role in peanut allergen stability and may determine immunodominant IgE-Binding epitopes. J Immunol 2002;169:882-7.
Flinterman AE, van Hoofen E, den Hartog Jager CF, Koppelman S, Pasmans SG, Hoekstra MO, Bruijnzeel-Koomen CA, Knulst AC, Knol EF. Children with peanut allergy recognize predominantly Ara h2 and Ara h 6, which remains stable over time. Clin Exp Allergy 2007;37:1221-8.
McDermott RA, Porterfield HS, El Mezayen R, Burks AW, Pons L, Schlichting DG, Solomon B, Redzic, JS, Harbeck RJ, Duncan MW, Hansen KC, Dreskin SC. Contribution of Ara h 2 to peanut-specific, immunoglobulin E-mediated, cell activation. Clin Exp Allergy 2007;37:752-763.