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| Allergen: | rApi g 1.0101 (Apium gravolens allergen 1.0101), celery | 
| Unit: | 250µg | 
| Source: | P. pastorsis | 
| Mol. Wt: | ~16 kD | 
| Purification: | Purified from Pichia pastoris culture by multi-step chromatography. Purity > 95% by silver stained SDS-PAGE, run under reducting condidtions. | 
| Concentration: | See product insert. | 
| Formulation: | Preservative and carrier free in 1X PBS buffer, pH 7.4 | 
| Storage: | Store at -20ºC. Aviod repeated freeze-thaw cycles. | 
| Notes: | (1)rApi g 1 has a C-terminal 6xHis-tag (2)Not tested for endotoxin. | 
| Product Resources: | Recombinant Api g 1 Certificate of Analysis | 
| Related products: | |
| Allergens are provided for research and commercial use in vitro: not for human in vivo or therapeutic use. | |
| References: | |
| Hoffman-Sommergruber K, Ferris R, Pec M, Radauer C, O’Riordain G, Laimer Da Camarara Machado M, et al. Characterization of Api g 1.0201, new member of the Api g 1 family of celery allergens. Int Arch Allergy Immunol 2000;122(2):115-23. | |
| Hoffmann-Sommergruber K, Demoly P, Crameri R, Breiteneder H, Ebner C, Laimer Da Camara Machado M, et al. IgE reactivity to Api g 1, a major celery allergen, in Central European population is based on primary sensitization by Bet v 1. J Allergy Clin Immunol 1999;104:478-84. | |
| Hoffman-Sommergruber K, Vanek-Krebitz M, Ferris R, O’Riordain G, Susani M, Hirschwehr R, et al. Isolation and cloning of Bet v 1-homologous food allergens from celeriac (Api g 1) and apple (Mal d 1). Adv Exp Med Biol 1996;409:219-24 | |
| Breitenender H, Hoffmann-Sommergruber K, O’Riordain G, Sasani M, Ahorn H, Ebner C, et al. Molecular characterization of Api g 1, the major allergen of celery (Apium graveolens), and its immunological and structural relationships to a group of 17-kDa free pollen allergens. Eur J Biochem 1995;(15)233:484-9. | |