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Recombinant Api g 1 (RP-AG1-1)

  • Recombinant Api g 1 (RP-AG1-1)
Allergen: rApi g 1.0101 (Apium gravolens allergen 1.0101), celery
Unit: 250µg
Source: P. pastorsis
Mol. Wt: ~16 kD
Purification: Purified from Pichia pastoris culture by multi-step chromatography. Purity > 95% by silver stained SDS-PAGE, run under reducting condidtions.
Concentration:

See product insert.

Formulation:

Preservative and carrier free in 1X PBS buffer, pH 7.4

Storage: Store at -20ºC. Aviod repeated freeze-thaw cycles.
Notes:

(1)rApi g 1 has a C-terminal 6xHis-tag

(2)Not tested for endotoxin.

   
Product Resources: Recombinant Api g 1 Certificate of Analysis
Related products:  
Allergens are provided for research and commercial use in vitro:  not for human in vivo or therapeutic use.
References:  
Hoffman-Sommergruber K, Ferris R, Pec M, Radauer C, O’Riordain G, Laimer Da Camarara Machado M, et al. Characterization of Api g 1.0201, new member of the Api g 1 family of celery allergens. Int Arch Allergy Immunol 2000;122(2):115-23.
Hoffmann-Sommergruber K, Demoly P, Crameri R, Breiteneder H, Ebner C, Laimer Da Camara Machado M, et al. IgE reactivity to Api g 1, a major celery allergen, in Central European population is based on primary sensitization by Bet v 1. J Allergy Clin Immunol 1999;104:478-84.
Hoffman-Sommergruber K, Vanek-Krebitz M, Ferris R, O’Riordain G, Susani M, Hirschwehr R, et al. Isolation and cloning of Bet v 1-homologous food allergens from celeriac (Api g 1) and apple (Mal d 1). Adv Exp Med Biol 1996;409:219-24
Breitenender H, Hoffmann-Sommergruber K, O’Riordain G, Sasani M, Ahorn H, Ebner C, et al. Molecular characterization of Api g 1, the major allergen of celery (Apium graveolens), and its immunological and structural relationships to a group of 17-kDa free pollen allergens. Eur J Biochem 1995;(15)233:484-9.
 
 
 
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  • Product Code: RP-AG1-1
  • Availability: In Stock
  • $625.00