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Recombinant Asp f 1.0101 (RP-AF1-1)

  • Recombinant Asp f 1.0101 (RP-AF1-1)
Allergen: rAsp f 1 (Aspergillus fumigatus allergen 1.0101), Common mold
Unit: 250µg
Source: Pichia pastoris
Mol. Wt: 18kD
Purification: Purified by multi-step HPLC. Purity on silver-stained SDS-PAGE >95%.
Concentration:

See product insert. 

Formulation:

Preservative-free and carrier-free in phosphate buffered saline, pH 7.4.  Filtered through 0.22µm filter.

Storage: Store at -20ºC. Avoid repeated Freeze/Thaw cycles.
Notes:

1) rAsp f 1 has a C-terminal 6x His-tag.

2) Upper band is Asp f 1 dimer.

Product Resources:

Recombinant Asp f 1 Certificate of Analysis

Allergens are provided for research and commercial use in vitro:  not for human in vivo or therapeutic use.
References:  
  1. Arruda, LK, Platts-Mills, TAE, Fox, JW and Chapman, MD (1990).  Aspergillus     fumigatus allergen I, a major IgE binding protein, is a member of the mitogillin family of cytotoxins.  J. Exp. Med., 172:1529-1532.
  2. Arruda LK, Platts-Mills TAE, Longbottom JL, Chapman MD (1992).  Aspergillus fumigatus: Identification of 16kd, 18kd and 45kd antigens recognized by human IgG and IgE antibodies and murine monoclonal antibodies.  J. Allergy Clin. Immunol. 89:1166-1176.
  3. Arruda LK, Mann BJ, Chapman MD (1992).  Selective expression of a major   allergen and cytotoxin, Asp f I, in Aspergillus fumigatus.  J. Immunol.149:3354-3359.
  4. Sporik RB, Arruda LK, Woodfolk J, Chapman MD, Platts-Mills TAE.   (1993).   Environmental exposure to Aspergillus fumigatus allergen 1 (Asp f I).   Clin Exp Allergy 23:326-331.
  5. El-Dahr JM, Fink R, Selden R, Arruda LK, Platts-Mills TAE, Heymann PW.  (1994).  Development of immune responses to Aspergillus at an early age in children with cystic fibrosis.  Am J Respir Crit Care Med 150:1513-1518.
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File Name Size Added Link
RP-AF1-1_CoA 34.72KB 11/10/2017 Download
Recombinant Asp f 1 Certificate of Analysis
  • Product Code: RP-AF1-1
  • Availability: In Stock
  • $625.00

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Tags: Purified Allergens, Mold Allergens, Recombinant Allergens,