Endotoxin Testing Food Sample Testing Lab Animal Allergen Testing Mass Spectrometry Testing Allergen Analysis Services
Allergens Food Protein Standards Antibodies ELISA 2.0 Kits Immunoassay Standards Human IgE mAb MARIA® Kits Molecular Reference Standards Ventia™ Apollo Air Sampler DUSTREAM® Air Sampling Cassettes Rhinovirus SARS-CoV-2 Proteins
Create Account Login View Cart
MENU ☰

Recombinant Bla g 4 (RP-BG4-1)

  • Recombinant Bla g 4 (RP-BG4-1)
Allergen: rBla g 4 (Blattella germanica allergen 4.0101), German cockroach
Unit: 250µg
Source: P. pastoris
Mol. Wt: 23kD
Purification: Purified from Pichia pastoris culture by phenol sepharose and gel filtration chromatography. Purity >95% by SDS-PAGE.
Concentration:

See product insert.
Formulation:

Preservative and carrier-free in phosphate buffered saline, pH 7.4. Filtered through 0.22 µm filter.
Storage: Store at -20ºC.
Notes: (1) rBla g 4 is produced in P. pastoris as a glycosylated allergen with MW slightly higher than that predicted for natural Bla g 4 (21kD). (2) Not tested for endotoxin.
Genbank: U40767
PDB: 3EBk, 3EBW, X-ray crystal structures
Product Resources: Recombinant Bla g 4 Certificate of Analysis
Allergens are provided for research and commercial use in vitro:  not for human in vivo or therapeutic use.
References:  
  1. Vailes LD, Kinter MT, Arruda LK, Chapman MD. High level expression of cockroach allergen, Bla g 4 in Pichia pastoris. J Allergy Clin Immunol 1998;101:274-80.
  2. Arruda LK, Vailes LD, Hayden ML, Benjamin DC, Chapman MD. Molecular cloning of cockroach allergen, Bla g 4, identifies ligand binding proteins (or calycins) as a cause of IgE antibody responses. J Biol Chem 1995;270:31196-210.
  3. Arruda LK, Vailes LD, Ferriani VPL, Santos ABR, Pomés A, Chapman MD. The role of cockroach allergens in asthma. J Allergy Clin Immunol 2001;107:419-28.
  4. Fan Y, Gore JC, Redding KO, Vailes LD, Chapman MD, Schal C. Tissue localization and regulation by juvenile hormone of human allergen Bla g 4 from the German cockroach, Blattella germanica (L.). Insect Mol Biol, 2005;14:45-53.
  5. Tan YW, Chan SL, Ong TC, Yit lY, Tiong YS, Chew FT, Sivaraman J, Mok YK. Structures of two major allergens, Bla g 4 and Per a 4, from cockroaches and their IgE binding epitopes. J Biol Chem, 2009;284:3148-3157.
File Name Size Added Link
RP-BG4_CoA 56.86KB 20/05/2014 Download
Recombinant Bla g 4 Certificate of Analysis
  • Product Code: RP-BG4-1
  • Availability: In Stock

  • $625.00