Recombinant Can f 2 (RP-CF2-1)

Allergen:	rCan f 2 (Canis familiaris allergen 2.0101), dog
Unit:	250µg
Source:	Pichia pastoris
Mol. Wt:	19 kD
Purification:	Purified from Pichia pastoris culture using His-Trap chromatography. Purity >95% on silver stained SDS-PAGE.
Concentration:	See Product Insert.
Formulation:	Preservative and carrier-free in phosphate buffered saline, pH 7.4. Filtered through 0.22µm filter.
Storage:	Store at -20ºC. Avoid repeated freeze-thaw cycles.
Notes:	(1) rCan f 2 presents as a 19kD unglycosylated and a 23 kD glycosylated monomer. In addition, a dimer around 40kD is visible under non-reducing conditions. (2) rCan f 2 has a C-terminal 6xHis-tag
Formulation:	Preservative and carrier-free in phosphate buffered saline, pH 7.4. Filtered through 0.22µm filter.
Product Resources:	Recombinant Can f 2 Certificate of Analysis
Allergens are provided for research and commercial use in vitro: not for human in vivo or therapeutic use.
References:
Konieczny A et al. The major dog allergens, Can f 1 and Can f 2, are salivary lipocalin proteins: cloning and immunological characterization of the recombinant forms. Immunology 1997; 92:577-86. Madhurantakam C et al. Crystal structure of the dog lipocalin allergen Can f 2: implications for cross-reactivity to the cat allergen Fel d 4. J. Mol. Biol. 2010; 401:68-83. Saarelainen S et al. Assessment of recombinant dog allergens Can f 1 and Can f 2 for the diagnosis of dog allergy. Clin Exp Allergy. 2004;34(10):1576-82.