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Recombinant Eur m 2 (RE-EM2-1)

  • Recombinant Eur m 2 (RE-EM2-1)
Allergen: rEur m 2.0101 (Euroglyphus maynei allergen 2)
Unit: 250µg
Source: E. coli
Mol. Wt: 14kD
Purification:

Purified from inclusion bodies using B-PER Buffer (Pierce). Solubilized rEur m 2 was refolded by dialysis into 20 mM Tris, 1mM EDTA, pH 8.5. Purity >95% by SDS-PAGE.
Concentration:

See product insert. 
Formulation:

20 mM Tris-HCl, pH 8.5, sterile filtered.
Storage: Store at -20ºC
Notes: (1) rEur m 2.0101 is suitable for T-cell or other in vitro studies, but does not react in the Group 2 mite ELISA . (2) Does react with anti-Group 2 mAb DpX which is used for specific IgE measurements in the chimeric ELISA. (3) Not tested for endotoxin.
Genbank:  
PDB:  
Product Resources: Recombinant Eur m 2 Certificate of Analysis
Allergens are provided for research and commercial use in vitro:  not for human in vivo or therapeutic use.
References:  
  1. Heyman PW, Chapman MD, Fox J, Aalberse, RC, PlattsMills, TAE. Antigenic and structural analysis of the Group II allergens (Derp II and Der f II) from house dust mites (Dermatophagoides sp). J Allergy Clin Immunology 1989;83:1055-67.
  2. Mueller GA, Smith AM, Williams DC Jr., Hakkaart GAJ, Aalberse RC, Chapman MD, Rule, GS, Benjmin DC. Expression and secondary structure determination by NMR methods of the major house dust mite allergen Der p 2. J Biol Chem 1997;272:26893-8.
  3. Smith AM, Benjamin DC, Hozic N, Derewenda U, Smith WA, Thomas WR, Gafvelin G, van Hage-Hamsten M, Chapman MD. The molecular basis of antigenic cross-reactivity between the group 2 mite allergens. J Allergy Clin Immunol 2001;107:977-84.
  4. Mueller GA, Smith AM, Chapman MD, Rule GS, Benjamin DC. Hydrogen exchange nuclear magnetic resonance spectroscopy mapping of antibody epitopes on the house dust mite allergen Der p 2. J. Biol. Chem. 2001;276:9359-65.
File Name Size Added Link
RE-EM2_CoA 33.74KB 20/05/2014 Download
Recombinant Eur m 2 Certificate of Analysis
  • Product Code: RE-EM2-1
  • Availability: In Stock

  • $625.00