Recombinant Ole e 1 (RP-OE1-1)

Allergen:	rOle e 1 (Olea europea allergen 1), olive
Unit:	250µg
Source:	Pichia pastoris
Mol. Wt:	~19kD and ~17kD. See silver stained SDS-PAGE gel on right.
Purification:	Purified from Pichia pastoris culture by HPLC. Purity >95% by SDS-PAGE.
Concentration:	See product insert.
Formulation:	Preservative-free and carrier-free in phosphate buffered saline, pH 7.4. Filtered through 0.22µm filter.
Storage:	Store at -20ºC. Avoid freeze-thaw cycles.
Notes:	1) rOle e 1 produced in Pichia pastoris has been observed to have two bands in the SDS-PAGE gel; a higher glycosylated band at ~19kD and a lower deglycosylated band at ~17kD. 2) Not tested for endotoxin.
Genbank:
PDB:
Product Resources:	Recombinant Ole e 1 Certificate of Analysis
Allergens are provided for research and commercial use in vitro: not for human in vivo or therapeutic use.
References:
Villalba M, Batanero E, Monsalve RI, de González la Peña MA, Lahoz C, Rodríguez R. Cloning and expression of Ole e 1, the major allergen from olive tree pollen. Polymorphism analysis and tissue specificity. J. Biol. Chem. 1994;269:15217-22. Hueccas S, Villalba M, González E, Martínez-Ruiz A, Rodríguez R. Production and detailed characterization of biologically active olive pollen allergen Ole e 1 secreted by the yeast Pichia pastoris. Eur. J. Biochem. 1999;261:539-545.

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Recombinant Ole e 1 Certificate of Analysis

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$665.00