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Allergen: | rSes i 1.0101 (Sesamum indicum allergen 1), sesame |
Unit: | 250µg |
Source: | P. pastoris |
Mol. Wt: | 16.4 kD |
Purification: | Purified from Pichia pastoris culture by multi-step column chromatography. Purity > 95% by silver stained SDS-PAGE. |
Concentration: |
See product insert. |
Formulation: |
Preservative and carrier free in 1X PBS buffer, pH 7.4. Filtered through 0.22µm filter. |
Storage: | Store at -20ºC. Aviod repeated freeze-thaw cycles. |
Notes: |
(1)rSes i 1 has a C-terminal 6xHis-tag |
Product Resources: | Recombinant Ses i 1 Certificate of Analysis |
Related products: | |
Allergens are provided for research and commercial use in vitro: not for human in vivo or therapeutic use. | |
References: | |
Sorgan S et al. Molecular Cloning of 11S Globulin and 2S Albumin, the Two Major Seed Storage Proteins in Sesame. J Agric Food Chem. 1999;4932-4938. | |
Pastorello EA et al. The Major Allergen of Sesame Seeds (Sesamum indicum) is a 2S Albumin. Journal of Chromatography B. 2001;756:85-93. | |
Wolff N et al. Allergy to Sesame in Humans is Associated Primarily with IgE Antibody to a 14 kDa 2S Albumin Precursor. Food and Chemical Toxicology. 2003;41:1165-1174. | |
Moreno JF et al. Thermostability and In Vitro Digestibility of a Purified Major Allergen 2S Albumin (Ses i 1) from White Sesame Seeds (Sesamum indicum L). Biochimica et Biophysica Acta. 2005;1752:142-153. | |