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Description: |
Identification of proteins in a gel band/spot or in solution. |
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Gel bands/Gel spots. We accept any type of gel matrix and most stains (please avoid the Bio-Rad Silver Stain Plus Kit). Make sure to cut the band close to your target, the gel band dimensions should be ~5mm x 2mm. |
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Steps: | ||||
Sample preparation: | Digestion with trypsin, Reduction & Alkylation | |||
LC-MS/MS: | Digested samples are analyzed with LC-MS/MS | |||
Data analysis: | Protein identification performed with Sequest HT | |||
Report generation: | PDF/Excel | |||
Turnaround time: | 2 weeks |