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Cor a 9 ELISA 2.0 kit - Twenty-five plate (EPC-CA9-25)

  • Cor a 9 ELISA 2.0 kit - Twenty-five plate (EPC-CA9-25)

ELISA 2.0 kit for measuring Corylus avellana allergen, Cor a 9.
Antibodies: 3B6/6F5
Standard Range: 100-0.2 ng/mL
Limit of Detection: 0.39 ng/mL
Background: OD<0.08 at 450nm
Coefficient of Determination: R-squared>0.98
Contents:

Plates: Pre-coated with anti-Cor a 9 monoclonal antibody 3B6
Vial 1: (white top) Cor a 9 allergen standard
Concentration: 1000 ng/ml
Vial 2: (brown) anti-Cor a 9 antibody 6F5
Vial 3: (blue top) Streptavidin-Peroxidase
Bottle 1: Wash buffer, (10x concentrate)
Bottle 2: Assay buffer, (10x concentrate)
Bottle 3: TMB developing substrate
Bottle 4: Stop solution (0.5N sulfuric acid)
Storage: The ELISA 2.0 kit should be stored at 2-8°C.
Expiry: See product insert.
Not Provided:

Type I ultrapure water or 18.2MΩ de-ionized water
Volumetric measuring equipment (e.g. serological pipette, graduated cylinder)
Clean containers for buffer and reagent preparation
Calibrated single and multi-channel micropipettes and tips
Vortex mixer
Plate reader capable of reading absorbance at 450nm
Analysis software (recommended, but not required)
Notes: The allergen standard is recommended for immunoassay calibration
purposes only.
A list of frequently asked questions and troubleshooting guide can be found under the ‘Support’ tab on our website.
Product Resources:

EPC-CA9-25 Certificate of Analysis

Validation Performance Data

For research and commercial use in vitro: not for human in vivo or therapeutic use.
  Certificate of Analysis
Pre-coated Plate: 96-well polystyrene microtiter plates coated with
monoclonal antibody 3B6 and treated with stabilizing agent. Sealed in foil pouch with desiccant.
Monoclonal Antibody: 3B6
Immunogen: Cor a 9
Isotype: Mouse IgG1/kappa
Specificity: Binds to species specific epitope present on Corylus avellana allergen, Cor a 9.
Purification: Produced in cell culture and purified by affinity chromatography using Protein A. Single heavy and light chain bands on SDS-PAGE.
  Certificate of Analysis
Detection Antibody: 6F5
Immunogen: Cor a 9
Isotype Mouse IgG2b/kappa
Specificity: Binds to species specific epitope present on Corylus avellana allergen, Cor a 9.
Purification: Produced in cell culture and purified by affinity chromatography using Protein A. Single heavy and light chain bands on SDS-PAGE.
Activity: Biotinylated and titrated for use in ELISA at 1/1000 dilution. Prepared in 1% BSA/50% glycerol/PBS, pH 7.4, 0.22 μm filtered, preservative free.
  Certificate of Analysis
Allergen Standard: Purified natural Cor a 9 prepared in 1% BSA/50%
glycerol/PBS, pH 7.4.
Concentration/Calibration: 1000 ng/mL (based on amino acid analysis)
References:  
 
 
 

 

 
File Name Size Added Link
EPC-CA9-X_CoA 1.16MB 28/04/2020 Download
Certificate of Analysis for Cor a 9 ELISA 2.0 kit
  • Product Code: EPC-CA9-25
  • Availability: In Stock

  • $9,095.00

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Tags: Cor a 9, ELISA 2.0, pre-coated plate,