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Shrimp Tropomyosin ELISA 2.0 kit - Twenty-five plate (EPC-TPM-25)
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ELISA 2.0 kit for measuring D. pteronyssinus tropomyosin allergen, Der p 10. |
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| Antibodies: | 1A6/pAb | ||||||
| Standard Range: | 50-0.1 ng/mL | ||||||
| Limit of Detection: | 0.2ng/mL | ||||||
| Background: | OD<0.05 at 450nm | ||||||
| Coefficient of Determination: | R-squared>0.98 | ||||||
| Contents: |
Plate: Pre-coated with anti-Tropomyosin monoclonal antibody 1A6 |
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| Storage: | The ELISA 2.0 kit should be stored at 2-8°C. | ||||||
| Expiry: | See product insert. | ||||||
| Not Provided: |
Type I ultrapure water or 18.2MΩ de-ionized water |
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| Notes: | The allergen standard is recommended for immunoassay calibration purposes only. A list of frequently asked questions and troubleshooting guide can be found under the ‘Support’ tab on our website. |
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| Product Resources: | |||||||
For research and commercial use in vitro: not for human in vivo or therapeutic use. |
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| Certificate of Analysis | |||||||
| Pre-coated Plate: | 96-well polystyrene microtiter plate coated with monoclonal antibody 1A6 and treated with stabilizing agent. Sealed in foil pouch with desiccant. |
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| Monoclonal Antibody: | 1A6 | ||||||
| Immunogen: | Mite (D. pteronyssinus) extract | ||||||
| Isotype: | Mouse IgG1 | ||||||
| Specificity: | Binds to specific epitope present on D. pteronyssinus tropomyosin allergen, Der p 10. Cross reactive with tropomyosin from multiple species. | ||||||
| Purification: | Produced in ascites and purified by chromatography using Protein A. Single heavy and light chain bands on SDS-PAGE. | ||||||
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| Certificate of Analysis | |||||||
| Detection Antibody: | Rabbit polyclonal antiserum | ||||||
| Immunogen: | Polyclonal rabbit antiserum raised against natural purified Shrimp Tropomyosin | ||||||
| Isotype | Rabbit polyclonal | ||||||
| Specificity: | The pAb contains IgG antibodies to shellfish tropomyosin | ||||||
| Activity: | The pAb is in phosphate buffered saline, pH 7.4, containing 1%BSA/50% glycerol. The pAb has been 0.22µm filtered and should be diluted 1/1000 for use in the Tropomyosin ELISA. | ||||||
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| Certificate of Analysis | |||||||
| Allergen Standard: | Purified natural Shrimp Tropomyosin prepared in 1% BSA/50% glycerol/PBS, pcH 7.4. |
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| Concentration/Calibration: | 500 ng/mL (based on amino acid analysis) | ||||||
| References: | |||||||
| 1. Shanti KN, Martin BM, Nagpal S, Metcalfe DD, Subba Rao PV. Identification of tropomyosin as the major shrimp allergen and characterization of its IgE-binding epitopes. J Immunol. 1993; 151 (10):5354-5363. | |||||||
| 2. Daul CB, Slattery M, Reese G, Lehrer SB. Identification of the major brown shrimp (Penaeus aztecus) allergen as the muscle protein tropomyosin. Int Arch Allergy Immunol. 1994; 105(1):49-55. | |||||||
| 3. Jeoung BJ, Reese G, Hauck P, Oliver JB, Daul CB, Lehrer SB. Quantification of the major brown shrimp allergen Pen a 1 (tropomyosin) by a monoclonal antibody-based sandwich ELISA. J Allergy Clin Immunol. 1997; 100(2):229-234. | |||||||
| 4. Santos AB, Rocha GM, Oliver C, Ferriani VPL, Lima RC, Palma MS, Sales VSF, Aalberse RC, Chapman MD, Arruda LK. Crossreactive IgE antibody responses to tropomyosins from Ascaris lumbricoides and cockroach. J Allergy Clin Immunol 2008; 121 (4):1040-1046. | |||||||
| File Name | Size | Added | Link |
| EPC-TPM-X COA | 266.57KB | 22/06/2023 | Download |
| EPC-TPM-X COA | |||
- Product Code: EPC-TPM-25
- Availability: In Stock
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$9,095.00
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Tags: Shrimp Tropomyosin, ELISA 2.0, pre-coated plate, five plates
