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Bet v 1 ELISA 2.0EP kit - Five plate pack (EPC-BV1-EP5)

  • Bet v 1 ELISA 2.0EP kit - Five plate pack (EPC-BV1-EP5)

ELISA 2.0EP kit for measuring Birch pollen allergen, Bet v 1

Cross-validated against the Candidate Standard Method for the European Pharmacopoeia

Antibodies: 5B4/6H4
Standard Range: 100-0.19 ng/ml
Limit of Detection: 0.39ng/ml
Background OD<0.08 at 450nm
Coefficient of Determination: R-squared>0.98
Contents: Plate: Pre-coated with anti-Bet v 1 monoclonal antibody 5B4
Vial 1: (white top) Bet v 1 allergen standard
Concentration: 1000 ng/ml
Vial 2: (brown) Biotinylated monoclonal antibody 6H4
Vial 3: (blue top) Streptavidin-peroxidase
Bottle 1: Wash buffer, (10x concentrate)
Bottle 2: Assay buffer, (10x concentrate)
Bottle 3: TMB developing substrate
Bottle 4: Stop solution (0.5N sulfuric acid)
Storage: Store at 2-8°C.
Expiry: See product insert.
Not Provided: Type I ultrapure water or 18.2MΩ de-ionized water
Volumetric measuring equipment (e.g. serological pipette, graduated cylinder)
Clean containers for buffer and reagent preparation
Calibrated single and multi-channel micropipettes and tips
Vortex mixer
Plate reader capable of reading absorbance at 450nm
Analysis software (recommended, but not required)
Notes: The allergen standard is recommended for immunoassay calibration
purposes only.
A list of frequently asked questions and troubleshooting guide can be found under the ‘Support’ tab on our website.
Product Resources:

EPC-BV1-EP5 Certificate of Analysis

Validation Performance Data

For research and commercial use in vitro: not for human in vivo or therapeutic use.

  Certificate of Analysis
Pre-coated Plate: 96-well polystyrene microtiter plate coated with
monoclonal antibody 4B10 and treated with stabilizing
agent. Sealed in foil pouch with desiccant.
Monoclonal Antibody: 5B4 (clone 5B4)
Immunogen: Birch pollen extract
Isotype: Mouse IgG1
Specificity: Recognizes distinct epitope on Betula verrucosa allergen, Bet v 1. Minimal cross-reactivity with PR-10 family of pollen allergens.
Purification: Produced in tissue culture and purified by affinity chromatography using Protein A. Single heavy and light chain bands on SDS-PAGE.

  Certificate of Analysis
Detection Antibody: 6H4 (clone 6H4)
Immunogen: Birch pollen extract
Isotype: Mouse IgG1
Specificity: Recognizes distinct epitope on Betula verrucosa allergen, Bet v 1. Minimal cross-reactivity with PR-10 family of pollen allergens.
Purification: Produced in tissue culture and purified by affinity chromatography using Protein A. Single heavy and light chain bands on SDS-PAGE.
Biotinylation: Biotinylated and titrated for use in ELISA at 1/1000 dilution. Prepared in 1% BSA/50% glycerol/PBS, pH 7.4, 0.22µm filtered, preservative free.

  Certificate of Analysis
Allergen Standard: Recombinant Bet v 1a prepared in 1% BSA/50%
glycerol/PBS, pH 7.4.
Concentration: 1000 ng/mL (Substandardized against the European Pharmacopoeia rBet v 1 CRS, Y0001565)

References:

  1. Kaul S, et al. Standardization of allergen products: 3. Validation of candidate European Pharmacopoeia standard methods for quantification of major birch allergen Bet v 1. Allergy. 2016; 71:1414.

  2. Vieths S, et al. Establishment of recombinant major allergens Bet v 1 and Phl p 5a as Ph. Eur. Reference standards and validation of ELISA methods for their measurement. Results from feasibility studies. Pharmeur Bio Sci Notes. 2012; 2012:118.

  3. Himly M, et al. Standardization of allergen products: 1. Detailed characterization of GMP-produced recombinant Bet v 1.0101 as biological reference preparation. Allergy. 2009; 64:1038.

File Name Size Added Link
EPC-BV1-EPX_CoA 1.17MB 28/04/2020 Download
Certificate of Analysis for Bet v 1 EP ELISA 2.0 kit
Spike Recovery Data 244.05KB 13/07/2023 Download
Spike Recovery Data
Validation Data 336.49KB 05/10/2023 Download
  • Product Code: EPC-BV1-EP5
  • Availability: In Stock
  • $2,345.00

Related Products

Bet v 1 EP(AS-BV1EP)
Natural Bet v 1 (NA-BV1-1)
Recombinant Bet v 1 (RP-BV1-1)

Tags: Bet v 1, ELISA 2.0EP. pre-coated plate, pollen, pollen allergen, Birch, European Pharmacopeia