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Allergen: | rPhl p 6 (Phleum pratense allergen 6.0101), Timothy |
Unit: | 250µg |
Source: | From Timothy grass pollen, expressed in Pichia pastoris |
Mol. Wt: | 10 kD; under reducing conditions |
Purification: | Purified from Pichia pastoris by multi-step HPLC. Purity > 95 % by silver stained SDS-PAGE. |
Concentration: | See product insert. |
Formulation: | Preservative-free and carrier-free in PBS, pH 7.4. Filtered through 0.22µm filter. |
Storage: | Store at -20ºC. Avoid repeated freeze-thaw cycles. |
Notes: | (1) Avoid repeated freeze-thaw cycles.
(2) rPhl p 6 has a C-terminal 6xHis-tag. |
Product Resources: | Recombinant Phl p 6 Certificate of Analysis |
Allergens are provided for research and commercial use in vitro: not for human in vivo or therapeutic use. | |
References: | |
1. Petersen A, Bufe A, Schlaak M, Becker WM. Characterization of the allergen group VI in timothy grass pollen (Phl p 6). I. Immunological and biochemical studies. Int Arch Allergy Immunol 1995, 108(1):49-54. 2. Vrtala S, Fisher S, Grote M, Vangelista L, Pastore A, Sperr WR, Valent P, Reichelt R, Kraft D, Valenta R. Molecular, immunological, and structural characterization of Phl p 6, a major allergen and P-particle-associated protein from Timothy grass (Phleum pretense) pollen. J Immunol 1999, 163:(10):5489-96. 3. Linhart B, Jahn-Schmid B, Verdino P, Keller W, Ebner C, Kraft D, Valenta R. Combination vaccines for the treatment of grass pollen allergy consisting of gentically engineered hybrid molecules with increased immunogenicity. FASEB J 2002, 16(10):1301-3. |