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Allergen: | rSin a 1 (Sinapis alba allergen 1.0101), Mustard |
Unit: | 250µg |
Source: | Pichia pastoris |
Mol. Wt: | 17 kD |
Purification: | Purified from Pichia pastoris culture by multi-step column chromatography. Purity > 95% by silver stained SDS-PAGE. |
Concentration: |
See product insert. |
Formulation: |
Preservative and carrier-free in 1X PBS, 0.3M NaCl, pH 7.4. Filtered through 0.22µm filter. |
Storage: | Store at -20ºC. Aviod repeated freeze-thaw cycles. |
Notes: |
(1)rSin a 1 has a C-terminal 6xHis-tag |
Product Resources: | Recombinant Sin a 1 Certificate of Analysis |
For Research Use Only: Not for Diagnostic or Therapeutic Use |
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References: |
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Menéndez-Arias L, Moneo I, Domínguez J, Rodríguez R. Primary Structure of the Major Allergen of Yellow Mustard (Sinapis alba L.) Seed, Sin a 1. Eur. J. Biochem. 1988;177:159-166. | |
González De La Peña MA, Monsalve RI, Batanero E, Villalba M, Rodríguez R. Expession in Escherichia coli of Sin a 1, the Major Allergen from Mustard. Eur. J. Biochem. 1996;237:827-832. | |
Palomares O, Cuesta-Herranz J, Rodríguez R, Villalba M. A Recombinant Precursor of the Mustard Allergen Sin a 1 Retains the Biochemical and Immunological Features of the Heterodimeric Native Protein. Int Arch Allergy Immunol 2005;137:18-26 | |