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Recombinant Bla g 2 (RP-BG2-1)

  • Recombinant Bla g 2 (RP-BG2-1)
Allergen: rBla g 2 (Blattella germanica allergen 2.0101), German cockroach
Unit: 250µg
Source: P. pastoris (clone NIG rBla g 2, glycosylation site N93Q mutated)
Mol. Wt: ~37 kD
Purification: Purified from Pichia pastoris culture supernatant by monoclonal antibody affinity chromatography. Purity by silver stained SDS-PAGE > 95%.
Concentration:

See product insert. 
Formulation:

Preservative and carrier-free in phosphate buffered saline, pH 7.4. Filtered through 0.22 µm filter.
Storage: Store at -20ºC.
Notes: (1) One glycosylation site was removed in this recombinant leaving two glycosylation sites. The recombinant is glycosylated and has a slightly higher MW than natural. Bla g 2 (36kD).
(2) This recombinant form was used to determine the crystal structure of Bla g 2.
Genbank: U28863 Mutation N93Q, substituted cag (Q) for aac (N)
PDB: 1YG9, 2NR6 (with Fab-7C11), X-ray crystal structures
Product Resources: Recombinant Bla g 2 Certificate of Analysis
Allergens are provided for research and commercial use in vitro:  not for human in vivo or therapeutic use.
References:
  1. Arruda LK, Vailes LD, Mann BJ, Shannon J, Fox JW, Vedvick TS, Hayden ML, Chapman MD. Molecular Cloning of a major cockroach (Blatella germanica) allergen, Bla g 2: Sequence homology to the aspartic proteases. J Biol Chem 1995;270:19563-8.
  2. Wünschmann S, Gustchina A, Chapman MD, Pomés A. Cockroach allergen Bla g 2: An unusual aspartic proteinase. J Allergy Clin Immunol 2005;116:140-5.
  3. Gustchina A, Li M, Wünschmann S, Chapman MD, Pomés A, Wodawer A. Crystal structure of cockroach allergen Bla g 2, an unusual zinc binding aspartic protease with a novel mode of self-inhibition. J Mol Biol 2005;348:433-44.
  4. Pomés A, Chapman MD, Vailes LD, Blundell TL, Venugopal D. Cockroach allergen Bla g 2: Structure function and implication for allergic sensitization. Amer J Resp Crit Care Med 2002;165:391-7.
  5. Li M, Gustchina A, Alexandratos J, Wlodawer A, Wünschmann S, Kepley CL, Chapman MD, Pomés A. Crystal structure of a dimerized cockroach allergen Bla g 2 complexed with a monoclonal antibody. J Biol Chem, 2008;283:22806-22814
File Name Size Added Link
RP-BG2_CoA 37.1KB 20/05/2014 Download
Recombinant Bla g 2 Certificate of Analysis
  • Product Code: RP-BG2-1
  • Availability: In Stock

  • $625.00

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