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Bla g 2 ELISA 2.0 kit - Single plate (EPC-BG2-1)
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ELISA 2.0 kit for measuring Blattella germanica allergen, Bla g 2. |
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| Antibodies: | 7C11/pAb | ||
| Standard Range: | 100-0.19 ng/mL | ||
| Limit of Detection: | 0.78ng/mL | ||
| Background: | OD<0.1 at 450nm | ||
| Coefficient of Determination: | R-squared>0.98 | ||
| Contents: |
Plate: Pre-coated with anti-Bla g 2 monoclonal antibody 7C11 |
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| Storage: | Store at 2-8°C | ||
| Expiry: | See product insert. | ||
| Not Provided: |
Type I ultrapure water or 18.2MΩ de-ionized water |
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| Notes: | The allergen standard is recommended for immunoassay calibration purposes only. A list of frequently asked questions and troubleshooting guide can be found under the ‘Support’ tab on our website |
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| Product Resources: | |||
For research and commercial use in vitro: not for human in vivo or therapeutic use. |
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| Certificate of Analysis | |||
| Pre-coated Plate: | 96-well polystyrene microtiter plate coated with monoclonal antibody 7C11 and treated with stabilizing agent. Sealed in foil pouch with desiccant |
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| Monoclonal Antibody: | 7C11 (Clone 7C11 C2 A6) | ||
| Immunogen: | Bla g 2 | ||
| Isotype: | Mouse IgG1 | ||
| Specificity: | Binds to an epitope on cockroach Blattella germanica allergen, Bla g 2. | ||
| Purification: | Produced in ascites and purified by ammonium sulphate precipitation and affinity chromatography using Protein A. Single heavy and light chain bands on SDS-PAGE. | ||
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| Certificate of Analysis | |||
| Detection Antibody: | Rabbit polyclonal antiserum | ||
| Immunogen: | Recombinant Bla g 2 | ||
| Isotype: | Multiple | ||
| Specificity: | The antiserum contains IgG antibodies to cockroach Blattella germanica allergen, Bla g 2 and does not react with Bla g 1. | ||
| Activity: | Titrated for use in ELISA at 1/1000 dilution. Prepared in 1% BSA/50% glycerol/PBS, pH 7.4, 0.22 μm filtered, preservative free. | ||
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| Certificate of Analysis | |||
| Allergen Standard: | Purified natural Bla g 2 prepared in 1% BSA/50% glycerol/PBS, pH 7.4. |
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| Concentration: | 1000 ng/mL (based on amino acid analysis) | ||
| References: | |||
| 1. Pollart SM, Smith TF, Morris E, Gelber LE, Platts-Mills TAE, Chapman MD. Environmental exposure to cockroach allergens: analysis with monoclonal Antibody based enzyme immunoassays. J Allergy Clin Immunol 87:505- 510, 1991. | |||
| 2. Gelber LE, Seltzer LH, Bouzoukis JK, Pollart SM, Chapman MD, Platts-Mills TAE. Sensitization ad exposure to indoor allergens as risk factors for asthma among patients presenting to hospital. Amer Rev Respir Dis 147:573-578, 1993. | |||
| 3. Rosenstreich DL, Eggleston P, Kattan M, Baker D, Slavin RG, Gergen P, Mitchell H, McNiff-Mortimer K, Lynn H, Ownby D, Malveaux F. The role of cockroach allergy and exposure to cockroach allergen in causing morbidity among inner-city children with asthma. New Eng J Med 336:1356-1363, 1997. | |||
| 4. Arruda LK, Vailes LD, Ferriani VP, Santos AB, Pomés A, Chapman MD. Cockroach allergens and asthma. J Allergy Clin Immunol 107:419-428, 2001. | |||
| 5. Pomés A, Vailes LD, Helm RM, Chapman MD. IgE reactivity of tandem repeats derived from cockroach allergen, Bla g 1. Eur J Biochem 269:3086-3092, 2002. | |||
| 6. Nalyanya G, Gore JC, Linker HM, Schal C. German cockroach allergen levels in North Carolina schools: comparison of integrated pest management and conventional cockroach control. J Med Entomol 46:420-427, 2009. | |||
| File Name | Size | Added | Link |
| EPC-BG2-X_CoA | 245.21KB | 19/02/2015 | Download |
| Certificate of Analysis for Bla g 2 ELISA 2.0 kit | |||
- Product Code: EPC-BG2-1
- Availability: In Stock
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$545.00
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Tags: bla g 2, ELISA 2.0, pre-coated plate, cockroach,
