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Per a 7 ELISA 2.0 kit - Five plate pack (EPC-PA7-5)
| ELISA 2.0 kit for measuring Periplaneta americana (tropomyosin) allergen, Per a 7. | ||||
| Antibodies: | pAb/1A6 | |||
| Standard Range: | 50.0 - 0.1 ng/ml | |||
| Limit of Detection: | 0.19ng/ml | |||
| Background | OD<0.08 at 450nm | |||
| Coefficient of Determination: | R-squared>0.98 | |||
| Contents: | Plate: Pre-coated with anti-shrimp tropomyosin polyclonal antibody Vial 1: (white top) Per a 7 allergen standard Concentration: 500 ng/ml Vial 2: (brown) Biotinylated monoclonal antibody 1A6 Vial 3: (blue top) Streptavidin-peroxidase Bottle 1: Wash buffer, (10x concentrate) Bottle 2: Assay buffer, (10x concentrate) Bottle 3: TMB developing substrate Bottle 4: Stop solution (0.5N sulfuric acid) |
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| Storage: | Store at 2-8°C. | |||
| Expiry: | See product insert. | |||
| Not Provided: | Type I ultrapure water or 18.2MΩ de-ionized water Volumetric measuring equipment (e.g. serological pipette, graduated cylinder) Clean containers for buffer and reagent preparation Calibrated single and multi-channel micropipettes and tips Vortex mixer Plate reader capable of reading absorbance at 450nm Analysis software (recommended, but not required) |
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| Notes: | The allergen standard is recommended for immunoassay calibration purposes only. A list of frequently asked questions and troubleshooting guide can be found under the ‘Support’ tab on our website. |
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| Product Resources: | ||||
For research and commercial use in vitro: not for human in vivo or therapeutic use. |
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| Certificate of Analysis | ||||
| Pre-coated Plate: | 96-well polystyrene microtiter plate coated with Rabbit polyclonal antiserum and treated with stabilizing agent. Sealed in foil pouch with desiccant. |
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| Polyclonal Antibody: | Rabbit polyclonal antiserum | |||
| Immunogen: | Polyclonal rabbit antiserum raised against natural purified Shrimp Tropomyosin | |||
| Isotype: | Multiple | |||
| Specificity: | The pAb contains IgG antibodies to shellfish tropomyosin and recognizes tropomyosin from a variety of animal species. | |||
| Purification: | Produced in acscites and purified by chromatography using Protein A. Prepared in phosphate buffered saline, pH 7.4, 0.22µm filtered, preservative free. | |||
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| Certificate of Analysis | ||||
| Detection Antibody: | 1A6 | |||
| Immunogen: | Mite (D. pteronyssinus) extract | |||
| Isotype: | Mouse IgG1 | |||
| Specificity: | Binds to a specific epitope present on Dermatophagoides pteronyssinus tropomyosin allergen, Der p 10. Cross reactive with tropomyosin from multiple species. | |||
| Purification: | Produced in ascites and purified by affinity chromatography using Protein A. Single heavy and light chain bands on SDS-PAGE. | |||
| Biotinylation: | Biotinylated and titrated for use in ELISA at 1/1000 dilution. Prepared in 1% BSA/50% glycerol/PBS, pH 7.4, 0.22µm filtered, preservative free. | |||
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| Certificate of Analysis | ||||
| Allergen Standard: | Recombinant Per a 7 prepared in 1% BSA/50% glycerol/PBS, pH 7.4. |
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| Concentration: | 500 ng/mL (based on amino acid analysis.) | |||
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References: |
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| File Name | Size | Added | Link |
| EPC-PA7-X_CoA | 1.06MB | 28/04/2020 | Download |
| Certificate of Analysis for Per a 7 ELISA 2.0 kit | |||
- Product Code: EPC-PA7-5
- Availability: In Stock
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$1,985.00
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Tags: Per a 7, ELISA 2.0. pre-coated plate, tropomyosin allergen, cockroach
