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Per a 7 ELISA 2.0 kit - Twenty-five plate pack (EPC-PA7-25)

  • Per a 7 ELISA 2.0 kit - Twenty-five plate pack (EPC-PA7-25)
ELISA 2.0 kit for measuring Periplaneta americana (tropomyosin) allergen, Per a 7.
Antibodies: pAb/1A6
Standard Range: 50.0 - 0.1 ng/ml
Limit of Detection: 0.19ng/ml
Background OD<0.08 at 450nm
Coefficient of Determination: R-squared>0.98
Contents: Plate: Pre-coated with anti-shrimp tropomyosin polyclonal antibody
Vial 1: (white top) Per a 7 allergen standard
Concentration: 500 ng/ml
Vial 2: (brown) Biotinylated monoclonal antibody 1A6
Vial 3: (blue top) Streptavidin-peroxidase
Bottle 1: Wash buffer, (10x concentrate)
Bottle 2: Assay buffer, (10x concentrate)
Bottle 3: TMB developing substrate
Bottle 4: Stop solution (0.5N sulfuric acid)
Storage: Store at 2-8°C.
Expiry: See product insert.
Not Provided: Type I ultrapure water or 18.2MΩ de-ionized water
Volumetric measuring equipment (e.g. serological pipette, graduated cylinder)
Clean containers for buffer and reagent preparation
Calibrated single and multi-channel micropipettes and tips
Vortex mixer
Plate reader capable of reading absorbance at 450nm
Analysis software (recommended, but not required)
Notes: The allergen standard is recommended for immunoassay calibration
purposes only.
A list of frequently asked questions and troubleshooting guide can be found under the ‘Support’ tab on our website.
Product Resources:

EPC-PA7-25 Certificate of Analysis

Validation Performance Data

For research and commercial use in vitro: not for human in vivo or therapeutic use.

  Certificate of Analysis
Pre-coated Plate: 96-well polystyrene microtiter plate coated with
Rabbit polyclonal antiserum and treated with stabilizing
agent. Sealed in foil pouch with desiccant.
Polyclonal Antibody: Rabbit polyclonal antiserum
Immunogen: Polyclonal rabbit antiserum raised against natural purified Shrimp Tropomyosin
Isotype: Multiple
Specificity: The pAb contains IgG antibodies to shellfish tropomyosin and recognizes tropomyosin from a variety of animal species.
Purification: Produced in acscites and purified by chromatography using Protein A. Prepared in phosphate buffered saline, pH 7.4, 0.22µm filtered, preservative free.

  Certificate of Analysis
Detection Antibody: 1A6
Immunogen: Mite (D. pteronyssinus) extract
Isotype: Mouse IgG1
Specificity: Binds to a specific epitope present on Dermatophagoides pteronyssinus tropomyosin allergen, Der p 10. Cross reactive with tropomyosin from multiple species.
Purification: Produced in ascites and purified by affinity chromatography using Protein A. Single heavy and light chain bands on SDS-PAGE.
Biotinylation: Biotinylated and titrated for use in ELISA at 1/1000 dilution. Prepared in 1% BSA/50% glycerol/PBS, pH 7.4, 0.22µm filtered, preservative free.

  Certificate of Analysis
Allergen Standard: Recombinant Per a 7 prepared in 1% BSA/50%
glycerol/PBS, pH 7.4.
Concentration: 500 ng/mL (based on amino acid analysis.)

References:

  1.  
File Name Size Added Link
EPC-PA7-X_CoA 1.06MB 28/04/2020 Download
Certificate of Analysis for Per a 7 ELISA 2.0 kit
  • Product Code: EPC-PA7-25
  • Availability: In Stock
  • $9,095.00

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Tags: Per a 7, ELISA 2.0. pre-coated plate, tropomyosin allergen, cockroach